Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have isolated a cDNA clone (SE5A) that encodes a protein on lampbrush chromosome loops of the newt Notophthalmus. In vitro-synthesized transcripts of this clone were injected into Xenopus oocytes, where they were efficiently translated. Most of the translated protein was imported into the oocytenucleus, and some of it appeared on the chromosome loops. The translation product must contain information that permits its appropriate targeting first to the nucleus and then to the chromosome loops.
Bürglin,
The nuclear migration signal of Xenopus laevis nucleoplasmin.
1987, Pubmed,
Xenbase
Bürglin,
The nuclear migration signal of Xenopus laevis nucleoplasmin.
1987,
Pubmed
,
Xenbase
Callan,
The lampbrush chromosomes of Xenopus laevis: preparation, identification, and distribution of 5S DNA sequences.
1987,
Pubmed
,
Xenbase
Dabauvalle,
Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
1982,
Pubmed
,
Xenbase
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Dingwall,
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
1988,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Forbes,
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
1983,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Lacroix,
Monoclonal antibodies to lampbrush chromosome antigens of Pleurodeles waltlii.
1985,
Pubmed
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Moreau,
Association of nucleoplasmin with transcription products as revealed by immunolocalization in the amphibian oocyte.
1986,
Pubmed
,
Xenbase
Roth,
Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes.
1987,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Scott,
Location of nuclear proteins on the chromosomes of newt oocytes.
1974,
Pubmed
Short,
Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
1988,
Pubmed
Theissen,
Cloning of the human cDNA for the U1 RNA-associated 70K protein.
1986,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
