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Mol Cell Biol
2000 Nov 01;2022:8590-601. doi: 10.1128/MCB.20.22.8590-8601.2000.
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The matrix protein of vesicular stomatitis virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore complexes.
Petersen JM
,
Her LS
,
Varvel V
,
Lund E
,
Dahlberg JE
.
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The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor of bidirectional nuclear transport. Here we demonstrate that inhibition occurs when M protein is in the nucleus of Xenopus laevis oocytes and that M activity is readily reversed by a monoclonal antibody (alphaM). We identify a region of M protein, amino acids 51 to 59, that is required both for inhibition of transport and for efficient recognition by alphaM. When expressed in transfected HeLa cells, M protein colocalizes with nuclear pore complexes (NPCs) at the nuclear rim. Moreover, mutation of a single amino acid, methionine 51, eliminates both transport inhibition and targeting to NPCs. We propose that M protein inhibits bidirectional transport by interacting with a component of the NPC or an NPC-associated factor that participates in nucleocytoplasmic transport.
Adam,
Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.
1994, Pubmed
Adam,
Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.
1994,
Pubmed
Ahmed,
Effect of vesicular stomatitis virus matrix protein on transcription directed by host RNA polymerases I, II, and III.
1998,
Pubmed
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000,
Pubmed
,
Xenbase
Black,
Vesicular stomatitis virus matrix protein inhibits host cell-directed transcription of target genes in vivo.
1992,
Pubmed
Black,
The role of vesicular stomatitis virus matrix protein in inhibition of host-directed gene expression is genetically separable from its function in virus assembly.
1993,
Pubmed
Chen,
Influenza A virus NS1 protein targets poly(A)-binding protein II of the cellular 3'-end processing machinery.
1999,
Pubmed
Coulon,
Genetic evidence for multiple functions of the matrix protein of vesicular stomatitis virus.
1990,
Pubmed
Dahlberg,
Functions of the GTPase Ran in RNA export from the nucleus.
1998,
Pubmed
Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed
Dobbelstein,
Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence.
1997,
Pubmed
Feldherr,
Signal-mediated nuclear transport in proliferating and growth-arrested BALB/c 3T3 cells.
1991,
Pubmed
Ferran,
The vesicular stomatitis virus matrix protein inhibits transcription from the human beta interferon promoter.
1997,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Follett,
Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus.
1974,
Pubmed
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fresco,
Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus.
1987,
Pubmed
Frishman,
Seventy-five percent accuracy in protein secondary structure prediction.
1997,
Pubmed
Frishman,
Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence.
1996,
Pubmed
Grimm,
In vivo selection of RNAs that localize in the nucleus.
1997,
Pubmed
,
Xenbase
Grimm,
Selection and nuclear immobilization of exportable RNAs.
1997,
Pubmed
,
Xenbase
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Görlich,
A novel class of RanGTP binding proteins.
1997,
Pubmed
,
Xenbase
Hammarskjöld,
Regulation of human immunodeficiency virus env expression by the rev gene product.
1989,
Pubmed
Her,
Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus.
1997,
Pubmed
,
Xenbase
Huber,
Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.
1998,
Pubmed
,
Xenbase
Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed
Jäkel,
Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells.
1998,
Pubmed
Kaptur,
Sequences of the vesicular stomatitis virus matrix protein involved in binding to nucleocapsids.
1991,
Pubmed
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Kutay,
Identification of a tRNA-specific nuclear export receptor.
1998,
Pubmed
,
Xenbase
Liu,
Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export.
1996,
Pubmed
Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Lyles,
Vesicular stomatitis virus M protein in the nuclei of infected cells.
1988,
Pubmed
Lyles,
Activity of vesicular stomatitis virus M protein mutants in cell rounding is correlated with the ability to inhibit host gene expression and is not correlated with virus assembly function.
1997,
Pubmed
Malim,
Mutational definition of the human immunodeficiency virus type 1 Rev activation domain.
1991,
Pubmed
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Moore,
Ran and nuclear transport.
1998,
Pubmed
Moore,
Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1.
1999,
Pubmed
,
Xenbase
Paik,
Inducible and conditional inhibition of human immunodeficiency virus proviral expression by vesicular stomatitis virus matrix protein.
1995,
Pubmed
Palacios,
Nuclear import of U snRNPs requires importin beta.
1997,
Pubmed
Pasquinelli,
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
1995,
Pubmed
,
Xenbase
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Rost,
PHD--an automatic mail server for protein secondary structure prediction.
1994,
Pubmed
Saavedra,
Regulation of mRNA export in response to stress in Saccharomyces cerevisiae.
1996,
Pubmed
Speckmann,
Nuclear retention elements of U3 small nucleolar RNA.
1999,
Pubmed
,
Xenbase
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Stanners,
Analysis of VSV mutant with attenuated cytopathogenicity: mutation in viral function, P, for inhibition of protein synthesis.
1977,
Pubmed
Takizawa,
Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta.
1999,
Pubmed
Talcott,
Getting across the nuclear pore complex.
1999,
Pubmed
Terns,
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.
1993,
Pubmed
,
Xenbase
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Ullman,
Nuclear export receptors: from importin to exportin.
1997,
Pubmed
Ullman,
The nucleoporin nup153 plays a critical role in multiple types of nuclear export.
1999,
Pubmed
,
Xenbase
Weis,
Importins and exportins: how to get in and out of the nucleus.
1998,
Pubmed
Wozniak,
Karyopherins and kissing cousins.
1998,
Pubmed
Yuan,
Inhibition of host RNA polymerase II-dependent transcription by vesicular stomatitis virus results from inactivation of TFIID.
1998,
Pubmed
