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Biochem J
2001 Dec 15;360Pt 3:691-8. doi: 10.1042/0264-6021:3600691.
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Phosphoinositide 3-kinase-gamma induces Xenopus oocyte maturation via lipid kinase activity.
Hehl S
,
Stoyanov B
,
Oehrl W
,
Schönherr R
,
Wetzker R
,
Heinemann SH
.
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Type-I phosphoinositide 3-kinases (PI3Ks) were characterized as a group of intracellular signalling proteins expressing both protein and lipid kinase activities. Recent studies implicate PI3Ks as mediators of oocyte maturation, but the molecular mechanisms are poorly defined. Here we used the Xenopus oocyte expression system as a model to investigate a possible contribution of the gamma-isoform of PI3K (PI3Kgamma) in the different pathways leading to cell-cycle progression by monitoring the time course of germinal vesicle breakdown (GVBD). Expression of a constitutive active PI3Kgamma (PI3Kgamma-CAAX) induced GVBD and increased the levels of phosphorylated Akt/protein kinase B and mitogen-activated protein kinase (MAPK). Furthermore, PI3Kgamma-CAAX accelerated progesterone-induced GVBD, but had no effect on GVBD induced by insulin. The effects of PI3Kgamma-CAAX could be suppressed by pre-incubation of the oocytes with LY294002, PD98059 or roscovitine, inhibitors of PI3K, MEK (MAPK/extracellular-signal-regulated protein kinase kinase) and cdc2/cyclin B kinase, respectively. Mutants of PI3Kgamma-CAAX, in which either lipid kinase or both lipid and protein kinase activities were altered or eliminated, did not induce significant GVBD. Our data demonstrate that expression of PI3Kgamma in Xenopus oocytes accelerates their progesterone-induced maturation and that lipid kinase activity is required to induce this effect.
Andersen,
Protein kinase B/Akt induces resumption of meiosis in Xenopus oocytes.
1998, Pubmed,
Xenbase
Andersen,
Protein kinase B/Akt induces resumption of meiosis in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Bayaa,
The classical progesterone receptor mediates Xenopus oocyte maturation through a nongenomic mechanism.
2000,
Pubmed
,
Xenbase
Bondeva,
Bifurcation of lipid and protein kinase signals of PI3Kgamma to the protein kinases PKB and MAPK.
1998,
Pubmed
Carnero,
Wortmannin, an inhibitor of phosphatidyl-inositol 3-kinase, induces oocyte maturation through a MPF-MAPK-dependent pathway.
1998,
Pubmed
,
Xenbase
Cork,
Second messenger signalling during hormone-induced Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Deuter-Reinhard,
SIP/SHIP inhibits Xenopus oocyte maturation induced by insulin and phosphatidylinositol 3-kinase.
1997,
Pubmed
,
Xenbase
Ferrell,
The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Ferrell,
Xenopus oocyte maturation: new lessons from a good egg.
1999,
Pubmed
,
Xenbase
Fisher,
Dissociation of MAP kinase activation and MPF activation in hormone-stimulated maturation of Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Flament,
Differential effects of 6-DMAP, olomoucine and roscovitine on Xenopus oocytes and eggs.
2000,
Pubmed
,
Xenbase
Grigorescu,
Insulin and IGF-1 signaling in oocyte maturation.
1994,
Pubmed
,
Xenbase
Guadagno,
Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Hu,
Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase.
1995,
Pubmed
,
Xenbase
King,
Mitosis in transition.
1994,
Pubmed
Klippel,
Membrane localization of phosphatidylinositol 3-kinase is sufficient to activate multiple signal-transducing kinase pathways.
1996,
Pubmed
Klippel,
A specific product of phosphatidylinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain.
1997,
Pubmed
Klippel,
Activation of phosphatidylinositol 3-kinase is sufficient for cell cycle entry and promotes cellular changes characteristic of oncogenic transformation.
1998,
Pubmed
Krugmann,
Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits.
1999,
Pubmed
Liu,
Molecular cloning of an amphibian insulin receptor substrate 1-like cDNA and involvement of phosphatidylinositol 3-kinase in insulin-induced Xenopus oocyte maturation.
1995,
Pubmed
,
Xenbase
Lopez-Ilasaca,
Linkage of G protein-coupled receptors to the MAPK signaling pathway through PI 3-kinase gamma.
1997,
Pubmed
Lutz,
G protein beta gamma subunits inhibit nongenomic progesterone-induced signaling and maturation in Xenopus laevis oocytes. Evidence for a release of inhibition mechanism for cell cycle progression.
2000,
Pubmed
,
Xenbase
López-Hernández,
Oncogenic Ras-induced germinal vesicle breakdown is independent of phosphatidylinositol 3-kinase in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Muslin,
Phosphatidylinositol 3-kinase activity is important for progesterone-induced Xenopus oocyte maturation.
1993,
Pubmed
,
Xenbase
Roche,
A function for phosphatidylinositol 3-kinase beta (p85alpha-p110beta) in fibroblasts during mitogenesis: requirement for insulin- and lysophosphatidic acid-mediated signal transduction.
1998,
Pubmed
Sadler,
Components of the signaling pathway linking the 1-methyladenine receptor to MPF activation and maturation in starfish oocytes.
1998,
Pubmed
,
Xenbase
Sheng,
Regulation of Xenopus oocyte meiosis arrest by G protein betagamma subunits.
2001,
Pubmed
,
Xenbase
Stephens,
The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101.
1997,
Pubmed
Stoyanov,
Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase.
1995,
Pubmed
Tian,
Identification of XPR-1, a progesterone receptor required for Xenopus oocyte activation.
2000,
Pubmed
,
Xenbase
Toker,
Signalling through the lipid products of phosphoinositide-3-OH kinase.
1997,
Pubmed
Vanhaesebroeck,
Signaling by distinct classes of phosphoinositide 3-kinases.
1999,
Pubmed
Vanhaesebroeck,
Autophosphorylation of p110delta phosphoinositide 3-kinase: a new paradigm for the regulation of lipid kinases in vitro and in vivo.
1999,
Pubmed
Wymann,
Structure and function of phosphoinositide 3-kinases.
1998,
Pubmed
Yamamoto-Honda,
Mutant of insulin receptor substrate-1 incapable of activating phosphatidylinositol 3-kinase did not mediate insulin-stimulated maturation of Xenopus laevis oocytes.
1996,
Pubmed
,
Xenbase
