XB-ART-31186
Cell
1981 Jan 01;231:89-93.
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Intranuclear location of the tRNA splicing enzymes.
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The intracellular location of the enzymes that excise tRNA intervening sequences and subsequently religate the molecular was investigated in Xenopus oocytes. All the splicing activity detected in a whole oocyte was present in the nucleus. By manual separation of the nuclear envelope and the nuclear contents (which form a gel-like structure in the presence of Mg++) it was found that all the activity of both splicing enzymes was located in the nuclear contents and was not associated with the nuclear membrane.
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Genes referenced: mt-tr trna