Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-8648
Mol Cell Endocrinol 2001 Jul 05;1811-2:97-110. doi: 10.1016/s0303-7207(01)00529-9.
Show Gene links Show Anatomy links

Expression of thyroid hormone receptor betaA gene assayed by transgenic Xenopus laevis carrying its promoter sequences.

Oofusa K , Tooi O , Kashiwagi A , Kashiwagi K , Kondo Y , Watanabe Y , Sawada T , Fujikawa K , Yoshizato K .


???displayArticle.abstract???
The responsiveness of thyroid hormone responsive element (TRE)-containing promoter sequence to thyroid hormone (TH) was studied utilizing Xenopus laevis carrying a transgene containing 5'-upstream region of TRbetaA1 gene and green fluorescent protein (EGFP) gene. EGFP-expression was seen first in neurulae, which continued to stage 45, then became weak, and again started to increase at the prometamorphic stage, culminating at the metamorphic climax stage. Immunohistochemistry identified eyes, viscera, and muscles as the EGFP-expressing larval tissues. The treatment of premetamorphic tadpoles with TH induced the precocious EGFP-expression. We also showed that the transgenic Xenopus adults were responsive to exogenous TH, a high responsiveness being seen in brain, small intestine, kidney, and bone. TRbeta-expression in the embryo, larva, and adult was verified by Western blotting. Thus, TH not only regulates the metamorphosis, but also might play some biological role(s) in embryos and adults.

???displayArticle.pubmedLink??? 11476944
???displayArticle.link??? Mol Cell Endocrinol


Species referenced: Xenopus laevis
Genes referenced: thrb