Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1994 Dec 20;9126:12477-81.
Show Gene links
Show Anatomy links
Nuclear assembly is independent of linker histones.
Dasso M
,
Dimitrov S
,
Wolffe AP
.
???displayArticle.abstract???
The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free extract of Xenopus eggs that transforms condensed sperm chromatin into DNA-replication-competent pronuclei. When linker histones were removed from the extract, the resultant pronuclei were indistinguishable from those formed in the complete extract. The assembly of functional nuclear membrane, nuclear lamina, and prereplication centers allowed identical DNA replication efficiencies. Thus, linker histones are not required for the assembly of morphologically normal nuclei capable of DNA replication.
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986, Pubmed,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bradbury,
Reversible histone modifications and the chromosome cell cycle.
1992,
Pubmed
Cook,
The nucleoskeleton and the topology of replication.
1991,
Pubmed
Dasso,
RCC1, a regulator of mitosis, is essential for DNA replication.
1992,
Pubmed
,
Xenbase
Dimitrov,
Chromatin transitions during early Xenopus embryogenesis: changes in histone H4 acetylation and in linker histone type.
1993,
Pubmed
,
Xenbase
Dimitrov,
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
1994,
Pubmed
,
Xenbase
Dworkin-Rastl,
The maternal histone H1 variant, H1M (B4 protein), is the predominant H1 histone in Xenopus pregastrula embryos.
1994,
Pubmed
,
Xenbase
Fairman,
Cellular factors required for multiple stages of SV40 DNA replication in vitro.
1988,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Grunstein,
Histone function in transcription.
1990,
Pubmed
Hansen,
Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III.
1992,
Pubmed
,
Xenbase
Hansen,
A role for histones H2A/H2B in chromatin folding and transcriptional repression.
1994,
Pubmed
,
Xenbase
Hock,
Absence of somatic histone H1 in oocytes and preblastula embryos of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Hozák,
Visualization of replication factories attached to nucleoskeleton.
1993,
Pubmed
Izaurralde,
Highly preferential nucleation of histone H1 assembly on scaffold-associated regions.
1989,
Pubmed
Kenny,
Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta.
1989,
Pubmed
Meier,
The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs.
1991,
Pubmed
,
Xenbase
Nakamura,
Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus.
1986,
Pubmed
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Newport,
A lamin-independent pathway for nuclear envelope assembly.
1990,
Pubmed
,
Xenbase
Ohsumi,
Chromosome condensation in Xenopus mitotic extracts without histone H1.
1993,
Pubmed
,
Xenbase
Pfaller,
Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro.
1991,
Pubmed
,
Xenbase
Roth,
Chromatin condensation: does histone H1 dephosphorylation play a role?
1992,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Smith,
Expression of a histone H1-like protein is restricted to early Xenopus development.
1988,
Pubmed
,
Xenbase
Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Wold,
Identification of cellular proteins required for simian virus 40 DNA replication.
1989,
Pubmed
Wolffe,
Replication timing and Xenopus 5S RNA gene transcription in vitro.
1993,
Pubmed
,
Xenbase
Wu,
Four distinct and unusual linker proteins in a mitotically dividing nucleus are derived from a 71-kilodalton polyprotein, lack p34cdc2 sites, and contain protein kinase A sites.
1994,
Pubmed
Wu,
An intervening sequence in an unusual histone H1 gene of Tetrahymena thermophila.
1986,
Pubmed
Zhao,
SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin.
1993,
Pubmed
