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EMBO J
1993 Aug 01;128:3133-42. doi: 10.1002/j.1460-2075.1993.tb05982.x.
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CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15.
Solomon MJ
,
Harper JW
,
Shuttleworth J
.
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The mitotic inducer p34cdc2 requires association with a cyclin and phosphorylation on Thr161 for its activity as a protein kinase. CAK, the p34cdc2 activating kinase, was previously identified as an enzyme necessary for this activating phosphorylation. We confirm here that CAK is a protein kinase and describe its purification over 13,000-fold from Xenopus egg extracts. We further show that CAK contains a protein identical or closely related to the previously identified Xenopus MO15 gene: p40MO15 copurifies with CAK, and an antiserum to p40MO15 specifically depletes cAK activity. CAK appears to be the only protein in Xenopus egg extracts that can activate complexes of either p34cdc2 or the closely related protein kinase, p33cdk2, with either cyclin A or cyclin B. The sequence similarity between p40MO15 and p34cdc2, and the approximately 200 kDa size of CAK, suggest that p40MO15 may itself be regulated by subunit association and by protein phosphorylations.
Colman,
Affinity labeling of purine nucleotide sites in proteins.
1983, Pubmed
Colman,
Affinity labeling of purine nucleotide sites in proteins.
1983,
Pubmed
Connell-Crowley,
Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro.
1993,
Pubmed
,
Xenbase
Desai,
Activation of human cyclin-dependent kinases in vitro.
1992,
Pubmed
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed
Elledge,
A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
1991,
Pubmed
,
Xenbase
Erikson,
Biochemical characterization of the p34cdc2 protein kinase component of purified maturation-promoting factor from Xenopus eggs.
1989,
Pubmed
,
Xenbase
Ershler,
Novel CDC2-related protein kinases produced in murine hematopoietic stem cells.
1993,
Pubmed
Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase
Gabrielli,
Cdc25 regulates the phosphorylation and activity of the Xenopus cdk2 protein kinase complex.
1992,
Pubmed
,
Xenbase
Galaktionov,
Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.
1991,
Pubmed
Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase
Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gu,
Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.
1992,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hata,
cDNA cloning of a novel cdc2+/CDC28-related protein kinase from rice.
1991,
Pubmed
Krek,
Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.
1991,
Pubmed
Lee,
cdc25+ encodes a protein phosphatase that dephosphorylates p34cdc2.
1992,
Pubmed
,
Xenbase
Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed
McGowan,
Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15.
1993,
Pubmed
Meijer,
Cyclin B targets p34cdc2 for tyrosine phosphorylation.
1991,
Pubmed
,
Xenbase
Meyerson,
A family of human cdc2-related protein kinases.
1992,
Pubmed
Millar,
p80cdc25 mitotic inducer is the tyrosine phosphatase that activates p34cdc2 kinase in fission yeast.
1991,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Nigg,
Vertebrate cdc2 kinase: its regulation by phosphorylation and its mitotic targets.
1991,
Pubmed
Norbury,
Animal cell cycles and their control.
1992,
Pubmed
Norbury,
Regulatory phosphorylation of the p34cdc2 protein kinase in vertebrates.
1991,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Paris,
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
1991,
Pubmed
,
Xenbase
Parker,
Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner.
1991,
Pubmed
Parker,
p107wee1 is a dual-specificity kinase that phosphorylates p34cdc2 on tyrosine 15.
1992,
Pubmed
Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed
Poon,
The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2.
1993,
Pubmed
,
Xenbase
Shuttleworth,
p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Simon,
KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28.
1986,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Solomon,
Role of phosphorylation in p34cdc2 activation: identification of an activating kinase.
1992,
Pubmed
,
Xenbase
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Solomon,
Activation of the various cyclin/cdc2 protein kinases.
1993,
Pubmed
Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed
Xiong,
Population explosion in the cyclin family.
1991,
Pubmed
Zoller,
Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.
1981,
Pubmed