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EMBO J
1992 Jan 01;111:335-43. doi: 10.1002/j.1460-2075.1992.tb05056.x.
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Roles of U4 and U6 snRNAs in the assembly of splicing complexes.
Vankan P
,
McGuigan C
,
Mattaj IW
.
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A series of U4 and U6 snRNA mutants was analysed in Xenopus oocytes to determine whether they block splicing complex assembly or splicing itself. All the U4 and U6 mutants found to be inactive in splicing complementation resulted in defects in assembly of either U4/U6 snRNP or of splicing complexes. No mutants were found to separate the entry of U5 and U6 snRNAs into splicing complexes and neither of these RNAs was able to associate with the pre-mRNA in the absence of U4. In the absence of U6 snRNA, however, U4 entered a complex containing pre-mRNA as well as the U1 and U2 snRNAs. U6 nucleotides whose mutation resulted in specific blockage of the second step of splicing in Saccharomyces cerevisiae are shown not to be essential for splicing in the oocyte assay. The results are discussed in terms of the roles of U4 and U6 in the assembly and catalytic steps of the splicing process.
Barabino,
Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly.
1990, Pubmed
Barabino,
Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly.
1990,
Pubmed
Behrens,
Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells.
1991,
Pubmed
Bindereif,
An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.
1987,
Pubmed
Bindereif,
Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.
1990,
Pubmed
Black,
U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.
1989,
Pubmed
Blencowe,
Antisense probing of the human U4/U6 snRNP with biotinylated 2'-OMe RNA oligonucleotides.
1989,
Pubmed
Bordonné,
Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo.
1990,
Pubmed
Brow,
Spliceosomal RNA U6 is remarkably conserved from yeast to mammals.
1988,
Pubmed
Brow,
Splicing a spliceosomal RNA.
1989,
Pubmed
Cech,
The generality of self-splicing RNA: relationship to nuclear mRNA splicing.
1986,
Pubmed
Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed
Cheng,
Spliceosome assembly in yeast.
1987,
Pubmed
Datta,
Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing.
1991,
Pubmed
Fabrizio,
In vitro assembly of yeast U6 snRNP: a functional assay.
1989,
Pubmed
Fabrizio,
Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing.
1990,
Pubmed
Grabowski,
Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome.
1986,
Pubmed
,
Xenbase
Guthrie,
Spliceosomal snRNAs.
1988,
Pubmed
Guthrie,
Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein.
1991,
Pubmed
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Hausner,
Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles.
1990,
Pubmed
Konarska,
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.
1986,
Pubmed
Konarska,
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
1987,
Pubmed
Lamm,
Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts.
1991,
Pubmed
Lamond,
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.
1988,
Pubmed
Legrain,
Early commitment of yeast pre-mRNA to the spliceosome pathway.
1988,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Madhani,
Multiple roles for U6 snRNA in the splicing pathway.
1990,
Pubmed
Newman,
Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage.
1991,
Pubmed
Okano,
Newly identified U4/U6 snRNP-binding proteins by serum autoantibodies from a patient with systemic sclerosis.
1991,
Pubmed
Pikielny,
Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
,
Pubmed
Ruby,
An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
1988,
Pubmed
Ruby,
Pre-mRNA splicing in yeast.
1991,
Pubmed
Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase
Seraphin,
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing.
1989,
Pubmed
Shannon,
Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.
1991,
Pubmed
Séraphin,
Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly.
1991,
Pubmed
Séraphin,
The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.
1991,
Pubmed
Tani,
The gene for the U6 small nuclear RNA in fission yeast has an intron.
1989,
Pubmed
Tani,
mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing.
1991,
Pubmed
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Wersig,
Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly.
1990,
Pubmed
Wu,
Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA.
1991,
Pubmed
Xu,
The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.
1990,
Pubmed
Zieve,
Cell biology of the snRNP particles.
1990,
Pubmed
