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Nuclear envelope assembly was studied in vitro using extracts from Xenopus eggs. Nuclear-specific vesicles bound to demembranated sperm chromatin but did not fuse in the absence of cytosol. Addition of cytosol stimulated vesicle fusion, pore complex assembly, and eventual nuclear envelope growth. Vesicle binding and fusion were assayed by light and electron microscopy. Addition of ATP and GTP to bound vesicles caused limited vesicle fusion, but enclosure of the chromatin was not observed. This result suggested that nondialyzable soluble components were required for nuclear vesicle fusion. GTP gamma S and guanylyl imidodiphosphate significantly inhibited vesicle fusion but had no effect on vesicle binding to chromatin. Preincubation of membranes with 1 mM GTP gamma S or GTP did not impair vesicle binding or fusion when assayed with fresh cytosol. However, preincubation of membranes with GTP gamma S plus cytosol caused irreversible inhibition of fusion. The soluble factor mediating the inhibition by GTP gamma S, which we named GTP-dependent soluble factor (GSF), was titratable and was depleted from cytosol by incubation with excess membranes plus GTP gamma S, suggesting a stoichiometric interaction between GSF and a membrane component in the presence of GTP gamma S. In preliminary experiments, cytosol depleted of GSF remained active for fusion of chromatin-bound vesicles, suggesting that GSF may not be required for the fusion reaction itself. We propose that GTP hydrolysis is required at a step before the fusion of nuclear vesicles.
Bacon,
The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.
1989, Pubmed
Bacon,
The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.
1989,
Pubmed
Bailly,
Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2.
1991,
Pubmed
Baker,
GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free protein transport reaction.
1990,
Pubmed
Balch,
Biochemistry of interorganelle transport. A new frontier in enzymology emerges from versatile in vitro model systems.
1989,
Pubmed
Beckers,
Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.
1989,
Pubmed
Beckers,
Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
1989,
Pubmed
Berrios,
Characterization of an ATPase/dATPase activity associated with the Drosophila nuclear matrix-pore complex-lamina fraction. Identification of the putative enzyme polypeptide by direct ultraviolet photoaffinity labeling.
1983,
Pubmed
Bigay,
Fluoroaluminates activate transducin-GDP by mimicking the gamma-phosphate of GTP in its binding site.
1985,
Pubmed
Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bourne,
Do GTPases direct membrane traffic in secretion?
1988,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Chavrier,
Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
1990,
Pubmed
Colman,
Meiotic maturation in Xenopus oocytes: a link between the cessation of protein secretion and the polarized disappearance of Golgi apparati.
1985,
Pubmed
,
Xenbase
Fischer von Mollard,
rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles.
1990,
Pubmed
Gilman,
G proteins: transducers of receptor-generated signals.
1987,
Pubmed
Gorvel,
rab5 controls early endosome fusion in vitro.
1991,
Pubmed
Goud,
Small GTP-binding protein associated with Golgi cisternae.
1990,
Pubmed
Goud,
A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast.
1988,
Pubmed
Hall,
The cellular functions of small GTP-binding proteins.
1990,
Pubmed
Hesketh,
Stimulated release of histamine by a rat mast cell line is inhibited during mitosis.
1984,
Pubmed
Kahn,
Fluoride is not an activator of the smaller (20-25 kDa) GTP-binding proteins.
1991,
Pubmed
Kornberg,
ATP utilization by rep protein in the catalytic separation of DNA strands at a replicating fork.
1978,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Lohka,
The reconstitution of nuclear envelopes in cell-free extracts.
1988,
Pubmed
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lucocq,
A mitotic form of the Golgi apparatus in HeLa cells.
1987,
Pubmed
Mayorga,
Regulatory role for GTP-binding proteins in endocytosis.
1989,
Pubmed
Melançon,
Involvement of GTP-binding "G" proteins in transport through the Golgi stack.
1987,
Pubmed
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Newmeyer,
Egg extracts for nuclear import and nuclear assembly reactions.
1991,
Pubmed
,
Xenbase
Newport,
The role of mitotic factors in regulating the timing of the midblastula transition in Xenopus.
1985,
Pubmed
,
Xenbase
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed
Newport,
A lamin-independent pathway for nuclear envelope assembly.
1990,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Orci,
Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.
1989,
Pubmed
Pfaller,
Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro.
1991,
Pubmed
,
Xenbase
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Pines,
p34cdc2: the S and M kinase?
1990,
Pubmed
Plutner,
Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway.
1990,
Pubmed
Rothman,
Movement of proteins through the Golgi stack: a molecular dissection of vesicular transport.
1990,
Pubmed
Rubins,
GTP-binding proteins in rat liver nuclear envelopes.
1990,
Pubmed
Seydel,
A 28,000-Da GDP/GTP-binding protein specific to the nuclear envelope.
1991,
Pubmed
Stearns,
ADP-ribosylation factor is functionally and physically associated with the Golgi complex.
1990,
Pubmed
Sternweis,
Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
1982,
Pubmed
Vigers,
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs.
1991,
Pubmed
,
Xenbase
Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase
Zeligs,
Mitosis in rat thyroid epithelial cells in vivo. I. Ultrastructural changes in cytoplasmic organelles during the mitotic cycle.
1979,
Pubmed