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EMBO J
1988 Jun 01;76:1605-14. doi: 10.1002/j.1460-2075.1988.tb02986.x.
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Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis.
Kleinschmidt JA
,
Seiter A
.
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The karyophilic protein N1 (590 amino acids) is an abundant soluble protein of the nuclei of Xenopus laevis oocytes where it forms defined complexes with histones H3 and H4. The amino acid sequence of this protein, as deduced from the cDNA, reveals a putative nuclear targeting signal as well as two acidic domains which are candidates for the interaction with histones. Using two different histone binding assays in vitro we have found that the deletion of the larger acidic domain reduces histone binding drastically to a residual value of approximately 15% of the complete molecule, whereas removal of the smaller acidic domain only slightly reduces histone complex formation in solution, but infers more effectively with binding to immobilized histones. In the primary structure of the protein both histone-binding domains are distant from the conspicuous nuclear accumulation signal sequence (residues 531-537) close to the carboxy terminus which is very similar to the SV40 large T-antigen nuclear targeting sequence. Using a series of N1 mutants altered by deletions or point mutations we show that this signal is required but not sufficient for nuclear accumulation of protein N1. The presence of an additional, more distantly related signal sequence in position 544-554 is also needed to achieve a level of nuclear uptake equivalent to that of the wild-type protein. Results obtained with point mutations support the concept of two nuclear targeting sequences and emphasize the importance of specific lysine and arginine residues in these signal sequences.
Ansorge,
Improved system for capillary microinjection into living cells.
1982, Pubmed
Ansorge,
Improved system for capillary microinjection into living cells.
1982,
Pubmed
Bonne-Andrea,
Rat liver HMG1: a physiological nucleosome assembly factor.
1984,
Pubmed
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Bürglin,
The nuclear migration signal of Xenopus laevis nucleoplasmin.
1987,
Pubmed
,
Xenbase
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Dabauvalle,
Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
1982,
Pubmed
,
Xenbase
Davey,
Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Dilworth,
Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts.
1987,
Pubmed
,
Xenbase
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Earnshaw,
Anionic regions in nuclear proteins.
1987,
Pubmed
Feldherr,
Evidence for mediated protein uptake by amphibian oocyte nuclei.
1983,
Pubmed
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Franke,
The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ.
1978,
Pubmed
Franz,
Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed
Hopp,
Prediction of protein antigenic determinants from amino acid sequences.
1981,
Pubmed
Horowitz,
Cytoplasmic exclusion as a basis for asymmetric nucleocytoplasmic solute distributions.
1976,
Pubmed
Isenberg,
Histones.
1979,
Pubmed
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Kleinschmidt,
High mobility group proteins of amphibian oocytes: a large storage pool of a soluble high mobility group-1-like protein and involvement in transcriptional events.
1983,
Pubmed
,
Xenbase
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Kreis,
Microinjection of fluorescently labeled proteins into living cells with emphasis on cytoskeletal proteins.
1982,
Pubmed
Kristofferson,
Direct observation of steady-state microtubule dynamics.
1986,
Pubmed
Krohne,
A major soluble acidic protein located in nuclei of diverse vertebrate species.
1980,
Pubmed
,
Xenbase
Krohne,
Immunological identification of the karyophilic, histone-binding proteins N1 and N2 in somatic cells and oocytes of diverse amphibia.
1985,
Pubmed
,
Xenbase
Lanford,
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
1984,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Lyons,
Pentapeptide nuclear localization signal in adenovirus E1a.
1987,
Pubmed
Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Moreland,
Amino acid sequences that determine the nuclear localization of yeast histone 2B.
1987,
Pubmed
Moreland,
Identification of a nuclear localization signal of a yeast ribosomal protein.
1985,
Pubmed
Paine,
Diffusive and nondiffusive proteins in vivo.
1984,
Pubmed
,
Xenbase
Pennings,
Limitations of the poly(glutamic acid) reconstitution method in the reassembly of mono- and dinucleosomes.
1986,
Pubmed
Peters,
Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
1984,
Pubmed
Richardson,
Nuclear location signals in polyoma virus large-T.
1986,
Pubmed
Roberts,
The effect of protein context on nuclear location signal function.
1987,
Pubmed
Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmidt-Zachmann,
A constitutive nucleolar protein identified as a member of the nucleoplasmin family.
1987,
Pubmed
,
Xenbase
Sealy,
Xenopus nucleoplasmin: egg vs. oocyte.
1986,
Pubmed
,
Xenbase
Stein,
Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.
1979,
Pubmed
Taylor,
The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
1985,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Walker,
The primary structures of non-histone chromosomal proteins HMG 1 and 2.
1980,
Pubmed
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Wychowski,
A domain of SV40 capsid polypeptide VP1 that specifies migration into the cell nucleus.
1986,
Pubmed
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase