Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Characterization of a stable, major DNA polymerase alpha species devoid of DNA primase activity.
Kaiserman HB
,
Benbow RM
.
???displayArticle.abstract???
We have purified from Xenopus laevis ovaries a major DNA polymerase alpha species that lacked DNA primase activity. This primase-devoid DNA polymerase alpha species exhibited the same sensitivity as the DNA polymerase DNA primase alpha to BuAdATP and BuPdGTP, nucleotide analogs capable of distinguishing between DNA polymerase delta and DNA polymerase DNA primase alpha. The primase-devoid DNA polymerase alpha species also lacked significant nuclease activity indicative of the alpha-like (rather than delta-like) nature of the DNA polymerase. Using a poly(dT) template, the primase-devoid DNA polymerase alpha species elongated an oligo(rA10) primer up to 51-fold more effectively than an oligo(dA10) primer. In direct contrast, the DNA polymerase DNA primase alpha complex showed only a 4.6-fold preference for oligoribonucleotide primers at the same template/primer ratio. The catalytic differences between the two DNA polymerase alpha species were most dramatic at a template/primer ratio of 300. The primase-devoid DNA polymerase alpha species was found at high levels throughout oocyte and embryonic development. This suggests that the primase-devoid DNA polymerase alpha species could play a physiological role during DNA chain elongation in vivo, even if it is chemically related to DNA polymerase DNA primase alpha.
Baril,
Action of pancreatic DNase: requirements for activation of DNA as a template-primer for DNA polymerase.
1977, Pubmed
Baril,
Action of pancreatic DNase: requirements for activation of DNA as a template-primer for DNA polymerase.
1977,
Pubmed
Bayne,
Isolation of circular viral and complementary strand DNA from bacteriophage f1 duplex replicative-form DNA.
1978,
Pubmed
Benbow,
DNA synthesis in a multi-enzyme system from Xenopus laevis eggs.
1978,
Pubmed
,
Xenbase
Benz,
Initiation of DNA replication by the dnaG protein.
1980,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Byrnes,
Structural and functional properties of DNA polymerase delta from rabbit bone marrow.
1984,
Pubmed
Campbell,
Eukaryotic DNA replication.
1986,
Pubmed
Conaway,
A DNA primase activity associated with DNA polymerase alpha from Drosophila melanogaster embryos.
1982,
Pubmed
Cotterill,
A cryptic proofreading 3'----5' exonuclease associated with the polymerase subunit of the DNA polymerase-primase from Drosophila melanogaster.
1987,
Pubmed
Crute,
Purification and characterization of two new high molecular weight forms of DNA polymerase delta.
1986,
Pubmed
DePamphilis,
Replication of eukaryotic chromosomes: a close-up of the replication fork.
1980,
Pubmed
Dresler,
2',3'-Dideoxythymidine 5'-triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis in human cells: evidence for involvement of DNA polymerase delta.
1987,
Pubmed
Gronostajski,
Purification of a primase activity associated with DNA polymerase alpha from HeLa cells.
1984,
Pubmed
Grosse,
Purification of a 9S DNA polymerase alpha species from calf thymus.
1981,
Pubmed
Hockensmith,
Kinetic characteristics which distinguish two forms of calf thymus DNA polymerase alpha.
1981,
Pubmed
Kaguni,
Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.
1983,
Pubmed
Kunkel,
Exonucleolytic proofreading by calf thymus DNA polymerase delta.
1987,
Pubmed
König,
Reactions in vitro of the DNA polymerase-primase from Xenopus laevis eggs. A role for ATP in chain elongation.
1983,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lamothe,
Accessory proteins for DNA polymerase alpha activity with single-strand DNA templates.
1981,
Pubmed
Lee,
Purification of deoxyribonucleic acid polymerase delta from calf thymus: partial characterization of physical properties.
1980,
Pubmed
Lee,
Differential inhibition of human placental DNA polymerases delta and alpha by BuPdGTP and BuAdATP.
1985,
Pubmed
Lee,
Human placental DNA polymerase delta: identification of a 170-kilodalton polypeptide by activity staining and immunoblotting.
1987,
Pubmed
Masaki,
10 S DNA polymerase alpha of calf thymus shows a microheterogeneity in its large polypeptide component.
1982,
Pubmed
Merril,
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
1981,
Pubmed
Murakami,
Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
1986,
Pubmed
Nelson,
Classification of DNA polymerase activities from ovaries of the frog, Xenopus laevis.
1983,
Pubmed
,
Xenbase
Plevani,
DNA polymerase I and DNA primase complex in yeast.
1984,
Pubmed
Pritchard,
Preparation of DNA polymerase alpha X C1C2 by reconstituting DNA polymerase alpha with its specific stimulatory cofactors, C1C2.
1983,
Pubmed
Pritchard,
DNA polymerase alpha cofactors C1C2 function as primer recognition proteins.
1983,
Pubmed
Rapaport,
HeLa cell DNA polymerase alpha is tightly associated with tryptophanyl-tRNA synthetase and diadenosine 5',5"'-P1,P4-tetraphosphate binding activities.
1981,
Pubmed
Rowen,
Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains.
1978,
Pubmed
Shioda,
DNA primase activity associated with DNA polymerase alpha from Xenopus laevis ovaries.
1982,
Pubmed
,
Xenbase
Skarnes,
Exonuclease activity associated with a multiprotein form of HeLa cell DNA polymerase alpha. Purification and properties of the exonuclease.
1986,
Pubmed
Suzuki,
Dissociation and reconstitution of a DNA polymerase alpha-primase complex.
1985,
Pubmed
Tanaka,
Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.
1982,
Pubmed
Tseng,
A DNA primase from mouse cells. Purification and partial characterization.
1983,
Pubmed
Vishwanatha,
A multiprotein form of DNA polymerase alpha from HeLa cells. Resolution of its associated catalytic activities.
1986,
Pubmed
Vishwanatha,
Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.
1986,
Pubmed
Wahl,
Properties of two forms of DNA polymerase delta from calf thymus.
1986,
Pubmed
Wang,
DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.
1984,
Pubmed
Wong,
Structural and enzymological characterization of immunoaffinity-purified DNA polymerase alpha.DNA primase complex from KB cells.
1986,
Pubmed
Yagura,
Mouse DNA polymerase accompanied by a novel RNA polymerase activity: purification and partial characterization.
1982,
Pubmed
Yagura,
Novel form of DNA polymerase alpha associated with DNA primase activity of vertebrates. Detection with mouse stimulating factor.
1983,
Pubmed
,
Xenbase
Yamaguchi,
DNA primase-DNA polymerase alpha from simian cells. Modulation of RNA primer synthesis by ribonucleoside triphosphates.
1985,
Pubmed
Zierler,
Stockpiling of DNA polymerases during oogenesis and embryogenesis in the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase
