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The subsynaptic 43-kDa protein is concentrated at developing nerve-muscle synapses in vitro.
Burden SJ
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A 43-kDa peripheral membrane protein is known to copurify with acetylcholine receptor (AcChoR)-rich membranes isolated from the electric organ of Torpedo californica. Immunoelectron microscopy and crosslinking studies have demonstrated that this 43-kDa protein is closely associated with the cytoplasmic domain(s) of the AcChoR and suggest that the 43-kDa protein could regulate the distribution of the AcChoR in the postsynaptic membrane. This paper demonstrates that this postsynaptic protein appears at developing neuromuscular synapses in Xenopus nerve/muscle cocultures as early as AcChoRs become clustered at synaptic sites. Moreover, this protein is concentrated at AcChoR clusters that occur on noninnervated muscle cells. The close spatial and temporal relationship of this subsynaptic protein and AcChoR clusters is consistent with a role for the 43-kDa protein in the formation and/or stabilization of AcChoR clusters.
Akers,
Promotion of retinal neurite outgrowth by substratum-bound fibronectin.
1981, Pubmed
Akers,
Promotion of retinal neurite outgrowth by substratum-bound fibronectin.
1981,
Pubmed
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Burden,
Crosslinking of proteins in acetylcholine receptor-rich membranes: association between the beta-subunit and the 43 kd subsynaptic protein.
1983,
Pubmed
Burden,
Identification of an intracellular postsynaptic antigen at the frog neuromuscular junction.
1982,
Pubmed
Elliott,
Purification of Torpedo californica post-synaptic membranes and fractionation of their constituent proteins.
1980,
Pubmed
Froehner,
Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.
1984,
Pubmed
Froehner,
Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.
1981,
Pubmed
Gysin,
Structural heterogeneity and subcellular distribution of nicotinic synapse-associated proteins.
1981,
Pubmed
Hamilton,
Formation of disulfide-linked oligomers of acetylcholine receptor in membrane from torpedo electric tissue.
1979,
Pubmed
Moody-Corbett,
Localization of cholinesterase at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve.
1981,
Pubmed
,
Xenbase
Neubig,
Acetylcholine and local anesthetic binding to Torpedo nicotinic postsynaptic membranes after removal of nonreceptor peptides.
1979,
Pubmed
Peng,
Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters in cultured muscle cells.
1985,
Pubmed
,
Xenbase
Porter,
Characterization and localization of the Mr = 43,000 proteins associated with acetylcholine receptor-rich membranes.
1983,
Pubmed
Sanes,
Brain extract induces synaptic characteristics in the basal lamina of cultured myotubes.
1984,
Pubmed
Sealock,
Ultrastructural localization of the Mr 43,000 protein and the acetylcholine receptor in Torpedo postsynaptic membranes using monoclonal antibodies.
1984,
Pubmed
Silberstein,
Aneural muscle cell cultures make synaptic basal lamina components.
1982,
Pubmed
Sobel,
Large-scale purification of the acetylcholine-receptor protein in its membrane-bound and detergent-extracted forms from Torpedo marmorata electric organ.
1977,
Pubmed
St John,
Nicotinic postsynaptic membranes from Torpedo: sidedness, permeability to macromolecules, and topography of major polypeptides.
1982,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wennogle,
Transmembrane orientation of proteins present in acetylcholine receptor-rich membranes from Torpedo marmorata studied by selective proteolysis.
1980,
Pubmed
