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Intermediate filaments in the Xenopus oocyte: the appearance and distribution of cytokeratin-containing filaments.
Godsave SF
,
Wylie CC
,
Lane EB
,
Anderton BH
.
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Cytokeratins have previously been shown to exist in the Xenopus oocyte cortex. Using three monoclonal antibodies against cytokeratins, we follow the appearance of cytokeratin-containing filaments, and their changes in distribution during oocyte differentiation and maturation. Cytokeratin-containing filaments are shown to change dramatically in distribution in the oocyte during its ontogeny.
Figs 1-2. Immunofluorescence labelling of frozen sections of Xenopus gut with anticytokeratin
antibodies. Phase contrast and fluorescence pairs are shown: Fig. 1A,B)
LP3K staining; Fig. 2A,B, LE65 staining. Bar = 50/mi.
Fig. 3. Immunofluorescence labelling of adult Xenopus gut with control tissue culture
medium. (A) phase contrast; (B) fluorescence. Bars = 50//m.
Figs 4-6. Immunofluorescence labelling of previtellogenic oocytes with antibodies
to cytokeratins. Bars = 20 (im. Fig. 4. Early previtellogenic oocyte from a frozen
section of ethanol-fixed ovary stained with LE65. (A) phase contrast; (B)
fluorescence. Fig. 5. Grazing section through the cortex of a previtellogenic oocyte
from a sample of unfixed frozen ovary, stained with LP1K. (A) phase contrast; (B)
fluorescence. Fig. 6. Previtellogenic oocyte exhibiting a mitochondrial cloud
(arrows) stained with LE65. (A) phase contrast; (B) fluorescence. Inset shows
mitochondrial cloud at twice the magnification of that in the parent figure. Note its
cortical shell of staining, and partial division into segments.
Figs 7-8. Immunofluorescence labelling of vitellogenic oocytes with antibodies to
cytokeratins. Bars = 50|ian. Fig. 7. Early vitellogenic oocyte stained with LP3K.
(A) phase contrast; (B) fluorescence, y, yolk platelets. Fig. 8. Mid-vitellogenic
oocyte stained with LE65. (A) phase contrast; (B) fluorescence.
Fig. 9. Immunofluorescence labelling of a vitellogenic oocyte with control tissue
culture medium. (A) phase contrast; (B) fluorescence. Bars = 50[im.
Figs 10-11. Immunofluorescence labelling of fully grown oocytes with LE65.
Bars = 50jian. Fig. 10. Animal hemisphere containing nucleus (n). (A) phase
contrast; (B) fluorescence. Fig. 11. Vegetal hemisphere. (A) phase contrast; (B)
fluorescence.
Fig. 12. Immunofluorescence labelling of the animal hemisphere of an unfertilized
egg using LP3K (A) and vegetal hemisphere of a fertilized egg using LE65 (B).
= 50/ifln.
Fig. 13. Nitrocellulose blotting of Xenopus tadpole protein with anti-IFA and
anti-cytokeratin monoclonal antibodies. (A) Triton-insoluble proteins stained with
anti-(intermediate filament antigen) antibodies (anti-IFA), track a; and LP1K anticytokeratin
antibodies, track b. (B) total tadpole protein stained with anti-IFA, track
a; and LE65 anti-cytokeratin antibodies, track b.
Fig. 14. Immunofluorescence labelling of cytokeratin in a section of an unfixed fully
grown oocyte using LP1K. Only the cortex and subcortical regions of the oocyte are
shown. Bar = 20/im.
