XB-ART-30224
Cell Differ
1983 May 01;125:257-64.
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The accumulation of the maternal pool of histone H1A during oogenesis in Xenopus laevis.
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The accumulation of the maternal pool of histone H1A in Xenopus laevis oocytes was measured by the use of a semi-quantitative immunoradiographic method. This method implies the size-fractionation of total basic protein extracts from oocytes on a polyacrylamide gel, blotting of the proteins to diazotized paper and detection of histone proteins on the blots with the aid of antibodies to histone H1A and of [125I]protein A. It is shown that the maternal H1A pool that is present in fully-grown oocytes is accumulated very early in oogenesis, mainly in early vitellogenic oocytes. Stage III oocytes (Dumont, 1972) already contain approximately 80% of the final amount of H1A that is found in mature oocytes. The minimum amount of histone H1A estimated to be present in mature oocytes is 8-10 ng.
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Species referenced: Xenopus laevis
Genes referenced: h1-3