Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Recruitment of N-CoR/SMRT-TBLR1 corepressor complex by unliganded thyroid hormone receptor for gene repression during frog development.
Tomita A
,
Buchholz DR
,
Shi YB
.
???displayArticle.abstract???
The corepressors N-CoR (nuclear receptor corepressor) and SMRT (silencing mediator for retinoid and thyroid hormone receptors) interact with unliganded nuclear hormone receptors, including thyroid hormone (T(3)) receptor (TR). Several N-CoR/SMRT complexes containing histone deacetylases have been purified. The best studied among them are N-CoR/SMRT complexes containing TBL1 (transducin beta-like protein 1) or TBLR1 (TBL1-related protein). Despite extensive studies of these complexes, there has been no direct in vivo evidence for the interaction of TBL1 or TBLR1 with TR or the possible involvement of such complexes in gene repression by any nuclear receptors in any animals. Here, we used the frog oocyte system to demonstrate that unliganded TR interacts with TBLR1 and recruits TBLR1 to its chromatinized target promoter in vivo, accompanied by histone deacetylation and gene repression. We further provide evidence to show that the recruitment of TBLR1 or related proteins is important for repression by unliganded TR. To investigate the potential role for TBLR1 complexes during vertebrate development, we made use of T(3)-dependent amphibian metamorphosis as a model. We found that TBLR1, SMRT, and N-CoR are recruited to T(3)-inducible promoters in premetamorphic tadpoles and are released upon T(3) treatment, which induces metamorphosis. More importantly, we demonstrate that the dissociation of N-CoR/SMRT-TBLR1 complexes from endogenous TR target promoters is correlated with the activation of these genes during spontaneous metamorphosis. Taken together, our studies provide in vivo evidence for targeted recruitment of N-CoR/SMRT-TBLR1 complexes by unliganded TR in transcriptional repression during vertebrate development.
Adams,
Genetic analysis of 29 kindreds with generalized and pituitary resistance to thyroid hormone. Identification of thirteen novel mutations in the thyroid hormone receptor beta gene.
1994, Pubmed
Adams,
Genetic analysis of 29 kindreds with generalized and pituitary resistance to thyroid hormone. Identification of thirteen novel mutations in the thyroid hormone receptor beta gene.
1994,
Pubmed
Alland,
Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression.
1997,
Pubmed
Amano,
Thyroid hormone regulation of a transcriptional coactivator in Xenopus laevis: implication for a role in postembryonic tissue remodeling.
2002,
Pubmed
,
Xenbase
Bassi,
X-linked late-onset sensorineural deafness caused by a deletion involving OA1 and a novel gene containing WD-40 repeats.
1999,
Pubmed
Brucker-Davis,
Genetic and clinical features of 42 kindreds with resistance to thyroid hormone. The National Institutes of Health Prospective Study.
1995,
Pubmed
Buchholz,
A dominant-negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes.
2003,
Pubmed
,
Xenbase
Burke,
Co-repressors 2000.
2000,
Pubmed
Chen,
Coactivation and corepression in transcriptional regulation by steroid/nuclear hormone receptors.
1998,
Pubmed
Chen,
A transcriptional co-repressor that interacts with nuclear hormone receptors.
1995,
Pubmed
Chen,
Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300.
1997,
Pubmed
Evans,
The steroid and thyroid hormone receptor superfamily.
1988,
Pubmed
Flamant,
Thyroid hormone receptors: lessons from knockout and knock-in mutant mice.
2003,
Pubmed
Fondell,
Unliganded thyroid hormone receptor inhibits formation of a functional preinitiation complex: implications for active repression.
1993,
Pubmed
Forrest,
Thyroid hormone receptor beta is essential for development of auditory function.
1996,
Pubmed
Forrest,
Recessive resistance to thyroid hormone in mice lacking thyroid hormone receptor beta: evidence for tissue-specific modulation of receptor function.
1996,
Pubmed
Forrest,
Deafness and goiter: molecular genetic considerations.
1996,
Pubmed
Fraichard,
The T3R alpha gene encoding a thyroid hormone receptor is essential for post-natal development and thyroid hormone production.
1997,
Pubmed
Furlow,
In vitro and in vivo analysis of the regulation of a transcription factor gene by thyroid hormone during Xenopus laevis metamorphosis.
1999,
Pubmed
,
Xenbase
Gauthier,
Different functions for the thyroid hormone receptors TRalpha and TRbeta in the control of thyroid hormone production and post-natal development.
1999,
Pubmed
Glass,
The coregulator exchange in transcriptional functions of nuclear receptors.
2000,
Pubmed
Guenther,
A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness.
2000,
Pubmed
Göthe,
Mice devoid of all known thyroid hormone receptors are viable but exhibit disorders of the pituitary-thyroid axis, growth, and bone maturation.
1999,
Pubmed
Hashimoto,
An unliganded thyroid hormone receptor causes severe neurological dysfunction.
2001,
Pubmed
Heinzel,
A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.
1997,
Pubmed
Hsia,
Involvement of chromatin and histone acetylation in the regulation of HIV-LTR by thyroid hormone receptor.
2001,
Pubmed
,
Xenbase
Hsia,
Chromatin disruption and histone acetylation in regulation of the human immunodeficiency virus type 1 long terminal repeat by thyroid hormone receptor.
2002,
Pubmed
,
Xenbase
Hu,
Transcriptional repression by nuclear hormone receptors.
2000,
Pubmed
Hörlein,
Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.
1995,
Pubmed
Ishizuka,
The N-CoR/histone deacetylase 3 complex is required for repression by thyroid hormone receptor.
2003,
Pubmed
Ishizuya-Oka,
Temporal and spatial regulation of a putative transcriptional repressor implicates it as playing a role in thyroid hormone-dependent organ transformation.
1997,
Pubmed
,
Xenbase
Ito,
Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.
1999,
Pubmed
Ito,
The TRAP/SMCC/Mediator complex and thyroid hormone receptor function.
2001,
Pubmed
Jepsen,
Biological roles and mechanistic actions of co-repressor complexes.
2002,
Pubmed
Jones,
Multiple N-CoR complexes contain distinct histone deacetylases.
2001,
Pubmed
,
Xenbase
Jones,
N-CoR-HDAC corepressor complexes: roles in transcriptional regulation by nuclear hormone receptors.
2003,
Pubmed
,
Xenbase
Kaneshige,
Mice with a targeted mutation in the thyroid hormone beta receptor gene exhibit impaired growth and resistance to thyroid hormone.
2000,
Pubmed
Kaneshige,
A targeted dominant negative mutation of the thyroid hormone alpha 1 receptor causes increased mortality, infertility, and dwarfism in mice.
2001,
Pubmed
Kawahara,
Developmental and regional expression of thyroid hormone receptor genes during Xenopus metamorphosis.
1991,
Pubmed
,
Xenbase
Laherty,
Histone deacetylases associated with the mSin3 corepressor mediate mad transcriptional repression.
1997,
Pubmed
Lazar,
Thyroid hormone receptors: multiple forms, multiple possibilities.
1993,
Pubmed
Li,
Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3.
2000,
Pubmed
,
Xenbase
Li,
Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression.
2002,
Pubmed
,
Xenbase
Machuca,
Analysis of structure and expression of the Xenopus thyroid hormone receptor-beta gene to explain its autoinduction.
1995,
Pubmed
,
Xenbase
Mangelsdorf,
The nuclear receptor superfamily: the second decade.
1995,
Pubmed
McKenna,
Nuclear receptor coregulators: cellular and molecular biology.
1999,
Pubmed
Nagy,
Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase.
1997,
Pubmed
Perlman,
Thyroid hormone nuclear receptor. Evidence for multimeric organization in chromatin.
1982,
Pubmed
Puzianowska-Kuznicka,
Both thyroid hormone and 9-cis retinoic acid receptors are required to efficiently mediate the effects of thyroid hormone on embryonic development and specific gene regulation in Xenopus laevis.
1997,
Pubmed
,
Xenbase
Rachez,
Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.
1999,
Pubmed
Rachez,
Mechanisms of gene regulation by vitamin D(3) receptor: a network of coactivator interactions.
2000,
Pubmed
Ranjan,
Transcriptional repression of Xenopus TR beta gene is mediated by a thyroid hormone response element located near the start site.
1994,
Pubmed
,
Xenbase
Refetoff,
The syndromes of resistance to thyroid hormone.
1993,
Pubmed
Rietveld,
In vivo repression of an erythroid-specific gene by distinct corepressor complexes.
2002,
Pubmed
Ryu,
The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1.
1999,
Pubmed
Sachs,
Dual functions of thyroid hormone receptors during Xenopus development.
2000,
Pubmed
,
Xenbase
Sachs,
Targeted chromatin binding and histone acetylation in vivo by thyroid hormone receptor during amphibian development.
2000,
Pubmed
,
Xenbase
Sachs,
Nuclear receptor corepressor recruitment by unliganded thyroid hormone receptor in gene repression during Xenopus laevis development.
2002,
Pubmed
,
Xenbase
Shi,
Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis.
2001,
Pubmed
Shi,
The earliest changes in gene expression in tadpole intestine induced by thyroid hormone.
1993,
Pubmed
,
Xenbase
Shi,
Cloning and characterization of the ribosomal protein L8 gene from Xenopus laevis.
1994,
Pubmed
,
Xenbase
Shi,
Tissue-dependent developmental expression of a cytosolic thyroid hormone protein gene in Xenopus: its role in the regulation of amphibian metamorphosis.
1994,
Pubmed
,
Xenbase
Tinnikov,
Retardation of post-natal development caused by a negatively acting thyroid hormone receptor alpha1.
2002,
Pubmed
Tomita,
Fusion protein of retinoic acid receptor alpha with promyelocytic leukemia protein or promyelocytic leukemia zinc finger protein recruits N-CoR-TBLR1 corepressor complex to repress transcription in vivo.
2003,
Pubmed
,
Xenbase
Tsai,
Molecular mechanisms of action of steroid/thyroid receptor superfamily members.
1994,
Pubmed
Underhill,
A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1.
2000,
Pubmed
Wen,
The histone deacetylase-3 complex contains nuclear receptor corepressors.
2000,
Pubmed
Wikström,
Abnormal heart rate and body temperature in mice lacking thyroid hormone receptor alpha 1.
1998,
Pubmed
Wong,
Coordinated regulation of and transcriptional activation by Xenopus thyroid hormone and retinoid X receptors.
1995,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Xu,
Coactivator and corepressor complexes in nuclear receptor function.
1999,
Pubmed
Yaoita,
Xenopus laevis alpha and beta thyroid hormone receptors.
1990,
Pubmed
,
Xenbase
Yaoita,
A correlation of thyroid hormone receptor gene expression with amphibian metamorphosis.
1990,
Pubmed
,
Xenbase
Yen,
Physiological and molecular basis of thyroid hormone action.
2001,
Pubmed
Yoon,
Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1.
2003,
Pubmed
Yoshizato,
Biochemistry and cell biology of amphibian metamorphosis with a special emphasis on the mechanism of removal of larval organs.
1989,
Pubmed
Zhang,
The N-CoR-HDAC3 nuclear receptor corepressor complex inhibits the JNK pathway through the integral subunit GPS2.
2002,
Pubmed
Zhang,
Differential expression of thyroid hormone receptor isoforms dictates the dominant negative activity of mutant Beta receptor.
2002,
Pubmed
Zhang,
The mechanism of action of thyroid hormones.
2000,
Pubmed