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Virol J
2008 May 19;5:60. doi: 10.1186/1743-422X-5-60.
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Alterations in intracellular potassium concentration by HIV-1 and SIV Nef.
Choi B
,
Fermin CD
,
Comardelle AM
,
Haislip AM
,
Voss TG
,
Garry RF
.
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HIV-1 mediated perturbation of the plasma membrane can produce an alteration in the transmembrane gradients of cations and other small molecules leading to cell death. Several HIV-1 proteins have been shown to perturb membrane permeability and ion transport. Xenopus laevis oocytes have few functional endogenous ion channels, and have proven useful as a system to examine direct effects of exogenously added proteins on ion transport. HIV-1 Nef induces alterations in the intracellular potassium concentration in CD4+ T-lymphoblastoid cells, but not intracellular pH. Two electrode voltage-clamp recording was used to determine that Nef did not form ion channel-like pores in Xenopus oocytes. These results suggest that HIV-1 Nef regulates intracellular ion concentrations indirectly, and may interact with membrane proteins such as ion channels to modify their electrical properties.
Figure 1. Alterations in intracellular K+ in T-lymphoblastoid cells incubated with recombinant HIV-1 and SIV Nef protein. H9 cells were loaded either with the K+ sensitive fluorescent indicated PBFI-AM (panel A) or the pH sensitive fluorescent indicator BCECF-AM (Panel B), then incubated with various concentrations of recombinant HIV-1 or SIV Nef protein for 15 min at 37°C, and fluorescence intensity was measured by using a fluorescence concentration analyzer. Each data point represents the mean ± standard error of eight independent determinations.
Figure 2. Fluorescent and light microscopic analysis of alteration in intracellular K+ concentrations in H9 cells treated with recombinant Nef. H9 cells were loaded with the fluorescent indicator PBFI-AM and incubated with 300 nM recombinant Nef protein or with control medium without Nef for 15 min at 37°C. Control cells (A and C) and cells incubated with Nef protein (B and D) were examined by light (A and B) and fluorescent (C and D) microscopy.
Figure 3. Membrane currents in Xenopus oocytes exposed to Nef protein. (A) 300 nM recombinant Nef, (B) control oocyte Panel C: current:voltage plots determined from data recorded in panel A and B.
Bubien,
HIV-gp120 activates large-conductance apamin-sensitive potassium channels in rat astrocytes.
1995, Pubmed
Bubien,
HIV-gp120 activates large-conductance apamin-sensitive potassium channels in rat astrocytes.
1995,
Pubmed
Carrasco,
Modification of membrane permeability by animal viruses.
1995,
Pubmed
Choi,
Role of potassium in human immunodeficiency virus production and cytopathic effects.
1998,
Pubmed
Coady,
Effects of Vpu expression on Xenopus oocyte membrane conductance.
1998,
Pubmed
,
Xenbase
Comardelle,
A synthetic peptide corresponding to the carboxy terminus of human immunodeficiency virus type 1 transmembrane glycoprotein induces alterations in the ionic permeability of Xenopus laevis oocytes.
1997,
Pubmed
,
Xenbase
Cooke,
Regulated expression vectors demonstrate cell-type-specific sensitivity to human immunodeficiency virus type 1 Nef-induced cytostasis.
1997,
Pubmed
Cooper,
Acute AIDS retrovirus infection. Definition of a clinical illness associated with seroconversion.
1985,
Pubmed
Costin,
Cytopathic mechanisms of HIV-1.
2007,
Pubmed
,
Xenbase
Costin,
Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein.
2007,
Pubmed
Curtain,
Cytotoxic activity of the amino-terminal region of HIV type 1 Nef protein.
1997,
Pubmed
Ewart,
The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels.
1996,
Pubmed
Fackler,
Association of human immunodeficiency virus Nef protein with actin is myristoylation dependent and influences its subcellular localization.
1997,
Pubmed
Fermin,
Membrane alterations linked to early interactions of HIV with the cell surface.
1992,
Pubmed
Fujii,
Soluble Nef antigen of HIV-1 is cytotoxic for human CD4+ T cells.
1996,
Pubmed
Garcia,
Serine phosphorylation-independent downregulation of cell-surface CD4 by nef.
1991,
Pubmed
Garry,
Cell surface effects of human immunodeficiency virus.
1988,
Pubmed
Garry,
Potential mechanisms for the cytopathic properties of HIV.
1989,
Pubmed
Gatti,
Inhibition of HIV type 1 production by hygromycin B.
1998,
Pubmed
Jamieson,
Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity.
1994,
Pubmed
Kestler,
Importance of the nef gene for maintenance of high virus loads and for development of AIDS.
1991,
Pubmed
Kort,
The nef protein of the human immunodeficiency virus type 1 (HIV-1) inhibits a large-conductance potassium channel in human glial cells.
1998,
Pubmed
Lamb,
Do Vpu and Vpr of human immunodeficiency virus type 1 and NB of influenza B virus have ion channel activities in the viral life cycles?
1997,
Pubmed
Macreadie,
Cytotoxicity resulting from addition of HIV-1 Nef N-terminal peptides to yeast and bacterial cells.
1997,
Pubmed
Makutonina,
Human immunodeficiency virus infection of T-lymphoblastoid cells reduces intracellular pH.
1996,
Pubmed
Nishimura,
Loss of naïve cells accompanies memory CD4+ T-cell depletion during long-term progression to AIDS in Simian immunodeficiency virus-infected macaques.
2007,
Pubmed
Piller,
Vpr protein of human immunodeficiency virus type 1 forms cation-selective channels in planar lipid bilayers.
1996,
Pubmed
Plymale,
Concentration-dependent differential induction of necrosis or apoptosis by HIV-1 lytic peptide 1.
1999,
Pubmed
Poggi,
Involvement of dihydropyridine-sensitive calcium channels in human dendritic cell function. Competition by HIV-1 Tat.
1998,
Pubmed
Rasheed,
Cell killing by ultraviolet-inactivated human immunodeficiency virus.
1986,
Pubmed
Schubert,
Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells.
1996,
Pubmed
,
Xenbase
Schwartz,
Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein.
1996,
Pubmed
Veazey,
Gastrointestinal tract as a major site of CD4+ T cell depletion and viral replication in SIV infection.
1998,
Pubmed
Voss,
Alteration of intracellular potassium and sodium concentrations correlates with induction of cytopathic effects by human immunodeficiency virus.
1996,
Pubmed
Werner,
HIV-1 Nef protein exhibits structural and functional similarity to scorpion peptides interacting with K+ channels.
1991,
Pubmed
