Reagents
. The suppliers we use are listed.
- Listing of Xenopus libraries available.
- Affigel 10; BioRad cat# 153-6046
- Affigel 15. Bio-Rad cat# 1536051. For a 1:1 mix of Affigel10 and 15 get cat#153-6098.
- Alkaline-phosphatase linked secondaries, horse anti-mouse Vector cat# AP-2000, goat anti-rabbit Vector Cat# AP-1000 ($65)
- antibiotic/antimycotic; 5 x 50 ml lyophilized. Sigma cat#A-7292
- antibodies: Developmental Studies Hybridoma Bank (DSHB)
- anti-digoxigenin antibodies; (AP linked Fab fragments) Boehringer cat# 1093274
- BCIP; 5-bromo-4-chloro-3indolyl-phosphate 50 mg/ml in DMF from Boehringer cat# 1383 221
- BM blocking agent, Boehringer cat# 1093088
- BM purple. Stable histochemical sunstrate. Boehringer cat #1442 074
- BSA; Sigma cat# A3350 (50 gm, fraction V)
- Cysteine hydrochloride. Sigma Cat# C-7880, (1 kg $170)
- Digoxigenin ribonucletides; DIG RNA labelling mix, 10X conc. Boehringer cat# 1277 073
- durcupan; kit, Fluka cat#44610
- EDTA; disodium salt. Sigma cat# (is there a reason we don't use the soluble tetra-sodium salt? No)
- Epoxy-Sepharose-6B; Pharmacia
- Ficoll; type 400 Sigma cat#F-4375
- Formalin; neutral buffered, 50 ml aliquots. Sigma cat# HT 50 1 4
- Formamide; molecular biology grade, Fisher cat#
- HEPES. Amersham Cat# USB 16926
- hCG, Sigma ca #CG-10
- HemoD clearing agent. A non-toxic (relatively) xylene substitute. Fisher cat# 15 182 507A (1 gallon $19)
- Herring Sperm DNA. Boehringer cat#223646
- Maleic acid. Sigma cat# M 0375
- NBT; 4 nitro blue tetrazolium chloride. Solution in 70% DMF from Boehringer cat # 1383 213
- nile blue; Sigma cat#N-5383
- neutral red, Sigma Cat# N 6634
- Orange substrate (HRP), HistoMark Orange, Kirkgaard and Perry Cat# 54 74 00
- Paraplast. Wax embedding material. Fisher cat #12-646-113. (8 x 1kg=$37)
- Permount. Sigma cat# SP15-500 (500 ml $20)
- propylene oxide. Fluka cat#82320 (1 litre $23)
- PVP (for Denhart's); Sigma cat# P-
- Secondary antibodies; AP linked, Vector labs (best). HRP, FITC and RITC linked Jackson.
- Thimerosal. Sigma Cat# T 5125 (10 gm $37)
- torula RNA; 5' to 3'
- triethanolamine (hydrochloride); Sigma T-1502
- tween-20; alias polyoxyethylene-sorbitan monolaurate. Sigma cat# P-1379
- red substrate; HistoMark Red kit from Kirkegaard and Perry Laboratories. Cat no. 55-69-00 $104. From Research Genetics cat#
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