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Xaml: Xenopus ortholgue of aml-1 (acute myelogenous leukemia associate gene), also known as CBF alpha-2, a member of the Runt related gene family (RDG, runt domain genes).

This page contains previously published text and images. Copyright © to all such materials belongs to the Company of Biologists (1998). Click on images to view enlargements (6K to 7K, < 2 seconds).

A. stage 14, ventral B. stage 17, dorsal C. stage 17, ventral D. stage 26 ventral E. stage 26, ventral, alpha T1 globin for comparison.
Dorsal view of Xaml expression in neuroblasts at stage 17. Ventral view of embryo shown in B; note that Xaml expression has expanded posteriorly. Ventral Xaml expression in a stage 26 embryo extends to the proctodeum (arrow). aT1-globin expression in a stage 26 embryo, has not yet reached the proctodeum (arrow) demonstrating that Xaml expression precedes a-globin in the VBI.

Graphical representation of Xaml expression, with Xaml shown in purple superimposed upon the gastrulation images of Keller (1991).

Cloned by: Original description:
Daniel Tracey et al., in Peter Gergen's laboratory Tracey et al. (1998) Development 125, 1371-1380.
Images submitted by: Sequence
assembled by Xenbase Genbank AF035446
Probe available from: Other papers by:
Peter Gergen W.D.Tracey or J.P.Gergen
In situ probe details: Xaml cDNA in BSK+ (Stratagene) Other papers on:
plasmid name: antisense RE cut: antisense transcription: Xaml or Xenopus CBF
p5.31 SalI T7  
mRNA generation
plasmid name: sense RE cut: sense transcription:
pCSX NotI SP6

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