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(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 17:19, 17 January 2012 Xenopus tropicalis and Xenopus laevis environmental parameter standard operating procedure (Kroll lab) (hist) [14,592 bytes] Virgilio (Talk | contribs) (Created page with "Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washinton University: [http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&v...")
- 16:43, 19 December 2011 Isolation of DNA from Red Blood Cells (Sive et al. 2000) (hist) [4,406 bytes] Virgilio (Talk | contribs) (Created page with "'''Information adapted from:''' ''Early Development of Xenopus Laevis: A Laboratory Manual.''[http://www.xenbase.org/literature/book.do?method=display&bookId=22] 2000; (First e...")
- 16:39, 19 December 2011 Extraction of DNA from Single Embryos (Sive et al. 2000) (hist) [2,373 bytes] Virgilio (Talk | contribs) (Created page with "'''Information adapted from:''' ''Early Development of Xenopus Laevis: A Laboratory Manual.''[http://www.xenbase.org/literature/book.do?method=display&bookId=22] 2000; (First e...")
- 16:38, 19 December 2011 Preparation of RNA (In VItro Transcription) (Sive et al. 2000) (hist) [8,067 bytes] Virgilio (Talk | contribs) (Created page with "'''Information adapted from:''' ''Early Development of Xenopus Laevis: A Laboratory Manual.''[http://www.xenbase.org/literature/book.do?method=display&bookId=22] 2000; (First e...")
- 16:37, 19 December 2011 Preparation of RNA Using Proteinase K/Li/Cl (Sive et al. 2000) (hist) [4,343 bytes] Virgilio (Talk | contribs) (Created page with "'''Information adapted from:''' ''Early Development of Xenopus Laevis: A Laboratory Manual.''[http://www.xenbase.org/literature/book.do?method=display&bookId=22] 2000; (First e...")
- 19:54, 16 December 2011 dejellying solution (Zorn lab) (hist) [633 bytes] Virgilio (Talk | contribs) (Created page with "Dejellying solution: 2% Cysteine-hydrochloride, pH 8 in 0.1xMBS, to remove the jelly coat from fertilised Xenopus eggs and embryos before they can be manipulated. {|border="1"...")
- 18:46, 16 December 2011 dejellying embryos (Sive et al. 2000) (hist) [1,891 bytes] Virgilio (Talk | contribs) (Created page with "'''Information adapted from:''' ''Early Development of Xenopus Laevis: A Laboratory Manual.''[http://www.xenbase.org/literature/book.do?method=display&bookId=22] 2000; (First e...")
- 16:04, 16 December 2011 DNA/RNA preparation (hist) [1,121 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :10x mRNA Transcription Buffer :2x mRNA Nucleotide Triphosphate Mix :TE :Homoginization Buffer :Reticulocyte Standard Buffer (RSB) :Nuclei Freezing Buffer ...")
- 14:23, 16 December 2011 microinjection (hist) [631 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :Ficoll, 2.5% :2.5% Ficoll in 1/3X MR (for use with X. laevis embryos) :3% Ficoll in 1/9XMR (for use with X.tropicalis embryos) '''Ficoll, 2.5%''' 2.5...")
- 15:00, 14 December 2011 embryo manipulation/dissection (hist) [997 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :Agarose Plates, 1% (for use with trops or laevis) :Dehydrating embryos :Rehydrating Embryos(for embryos stored in 100% methanol) '''Agarose Plates, 1% ...")
- 20:47, 12 December 2011 general buffers and salt solutions (hist) [1,698 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :HEPES 1 M, pH 7.4 :1M MgCl2 :10x Phosphate Buffered Saline (PBS) :20x Phosphate Buffered Saline (PBS) :PBT :Proteinase K(PK), 10 mg/ml :PTw :4M NaCl, 1 li...")
- 20:42, 12 December 2011 fixatives (hist) [1,653 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :MEMFA :Bouin’s Fixative :Dent's Fix (Methanol/DMSO) :Paraformaldehyde(PFA), 20% :PTw Containing 4% Paraformaldehyde '''MEMFA''' (MOPS/EGTA/Magnesium S...")
- 20:39, 12 December 2011 in situ hybridization (hist) [6,390 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :Nucleotide Stocks :5x SP6 Buffer :MEMFA :PTw :Triethanolamine 0.1 M :PTw Containing 4% Paraformaldehyde :Hybridization Buffer :SSC, 20X :Denhardt’s solu...")
- 20:32, 12 December 2011 immunohistochemistry (hist) [2,145 bytes] Virgilio (Talk | contribs) (Created page with "'''In this section:''' :MEMFA :Dent's Fix (Methanol/DMSO) :Bleaching solution (Mayor et al. 1995) :Goat Serum (GS) :PBT :DAB :H2O2 :Secondary Antibodies Coupled to Horseradish P...")
- 15:16, 12 December 2011 culture media (hist) [4,353 bytes] Virgilio (Talk | contribs) (Created page with "Culture Media '''Modified Barth's Saline (MBS)''' Prepare two solutions: 0.1M CaCl2 and 10X MPS salts (Nacl/KCl/MgSO4/HEPES/NaHCO3) 0.1M Cacl2 :11.1 g/liter :autoclave and st...")
- 17:59, 6 December 2011 recipes for miscellaneous salts, solutions, etc. (Khokha lab) (hist) [7,994 bytes] Virgilio (Talk | contribs) (Created page with "'''Modified Ringers''' 1X Mol. Wt. 10X for 1L 10X* For 10L 10X For 20L 10X NaCl 0.1M 58.44 1 M 58.44 g 584.4 g 1168.8 g KCL 1.8 mM 74.56 18 mM 1.34 g 13.4 g ...")
- 17:00, 6 December 2011 benzocaine stock, 100x (Khokha lab) (hist) [317 bytes] Virgilio (Talk | contribs) (Created page with "submitted by VGP from Khokha lab protocols [http://khokha.medicine.yale.edu/protocols_general/Protocol_100XBenzocaine/protocol_100XBenzocaine.html] 100X Benzocaine Sigma Cat ...")
- 16:52, 6 December 2011 Lab Solutions and Reagents (hist) [2,582 bytes] Virgilio (Talk | contribs) (Created page with "**high-salt solution for egg laying (Zorn lab/Ron Laskey)")
- 15:24, 23 November 2011 high-salt solution for egg laying (Zorn lab) (hist) [1,420 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP RAL’s Ca++-free High Salt Barth: Egg Laying (8X) ___________________(1x solution)___________________________amounts to make 8X stock__________ __...")
- 19:49, 22 November 2011 hydrochloric acid antigen retrieval (Zorn lab) (hist) [1,334 bytes] Virgilio (Talk | contribs) (Created page with "protocol submitted by VGP Hydrochloric Acid (HCl) Antigen Retrieval Protocol Solutions and Reagents: Hydrochloric Acid (HCl) Solution (2N in Distilled Water, pH 0.6-0.9...")
- 19:45, 22 November 2011 dejellying embryos (Zorn lab) (hist) [1,511 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP Dejellying solution: 2% Cysteine-hydrochloride, pH 8 in 0.1xMBS, to remove the jelly coat from fertilised Xenopus eggs and embryos before they can be...")
- 19:12, 22 November 2011 anesthesia, in vitro fertilization, and natural mating of Xenopus tropicalis (Conlon lab) (hist) [6,580 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/TropIV...")
- 19:00, 22 November 2011 tadpole tail sampling for genomic DNA (Conlon lab) (hist) [2,270 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Tadpol...")
- 18:54, 22 November 2011 western blot protocol (Conlon lab) (hist) [3,962 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Wester...")
- 18:43, 22 November 2011 SDS-PAGE gels (Conlon lab) (hist) [5,462 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/SDS_pag...")
- 16:18, 22 November 2011 whole embryo protein extraction with freon (Conlon lab) (hist) [1,446 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Whole%...")
- 15:45, 22 November 2011 TUNEL staining of whole Xenopus embryos - Harland protocol (Conlon lab) (hist) [2,630 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/TUNEL%...")
- 15:17, 22 November 2011 transgenesis in Xenopus laevis - adapted from Amaya et al 2001 (Conlon lab) (hist) [18,919 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Transg...")
- 14:50, 22 November 2011 in vitro fertilization of Xenopus laevis (Conlon lab) (hist) [1,167 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/in%20v...")
- 14:39, 22 November 2011 benzidine-peroxidase staining for blood (Conlon lab) (hist) [634 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Benzid...")
- 14:31, 22 November 2011 immunostaining sectioned embryos (Conlon lab) (hist) [1,723 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Immuno...")
- 19:59, 18 November 2011 cryosectioning Xenopus embryos (Conlon lab) (hist) [1,707 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Cryose...")
- 19:49, 18 November 2011 wholemount antibody staining - adapted from Dan Weeks' protocol (Conlon lab) (hist) [1,711 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Weeks%...")
- 16:07, 18 November 2011 wholemount antibody staining (Conlon lab) (hist) [1,259 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Whole-...")
- 14:52, 18 November 2011 in situ checklist (Conlon lab) (hist) [1,272 bytes] Virgilio (Talk | contribs) (Created page with "Protocol checklist submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol checklist word document [http://www.unc.edu/...")
- 14:48, 18 November 2011 whole-mount in situ hybridization (Conlon lab) (hist) [9,048 bytes] Virgilio (Talk | contribs) (Created page with "Protocol submitted by VGP from Conlon lab protocols [http://www.unc.edu/~fconlon/Protocols.htm#tropprot] link to protocol document [http://www.unc.edu/~fconlon/Protocols/Whole-...")
- 16:54, 14 November 2011 Immunofluorescence with biotinylated antibodies (hist) [2,424 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 16:11, 14 November 2011 Silver staining protein gels (hist) [1,562 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:57, 14 November 2011 Immunoprecipitation (hist) [3,532 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:50, 14 November 2011 In-vitro kinetochore-microtubule binding assays (hist) [2,286 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:45, 14 November 2011 Assaying H1 kinase in extracts (hist) [2,411 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:31, 14 November 2011 Isolation of mitotic chromosomes from XTC cells (hist) [2,785 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:14, 14 November 2011 Cycling Extract (3) (hist) [2,070 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:11, 14 November 2011 Cycling Extract (2) (hist) [2,557 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 15:08, 14 November 2011 CSF Extract (2) (hist) [2,062 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 14:57, 14 November 2011 CSF Extract (1) (hist) [4,609 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 20:59, 11 November 2011 Tissue culture of Xenopus cell lines S3, XTC (hist) [3,053 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from Stukenberg lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1311&tabId=0] link to protocol page [http://people.v...")
- 20:11, 11 November 2011 Preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase) (hist) [2,399 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from David Shechter lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1810&tabId=0] Link to PDF: [http://www.shechterl...")
- 19:39, 11 November 2011 Isolation of chromatin from egg extract and histone recovery (hist) [1,904 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from David Shechter lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1810&tabId=0] Link to PDF: [http://www.shechterl...")
- 19:33, 11 November 2011 Crude interphase egg extract ("LSS" - low speed supernatant) (hist) [5,706 bytes] 205.142.197.33 (Talk) (Created page with "Protocol submitted by VGP from David Shechter Lab protocols [http://www.xenbase.org/community/person.do?method=display&personId=1810&tabId=0] Link to PDF: [http://www.shechterl...")