Xenbase Image ID: 133204

Fig. 5. Proximal Tubule Phenotype upon Inhibition of Cholesterol Synthesis. (A–B׳) Immunofluorescence analysis of untreated controls and embryos treated with 125 μM Mevinolin at stage 40 using anti-Caveolin-1 (red) antibody and ECL (green) with the gray-scale image showing Caveolin-1 staining alone and the color panels showing merged images. DAPI (blue) was used to visualize nuclei. (C) Quantification of the number of Caveolin-1-positive foci identified in (A,B). The number of embryos analyzed is indicated in the individual bars. Data were analyzed by Student׳s t-Test and asterisk represent a significance of p<0.05. (D) Bar diagram of the number of proximal tubular cells in untreated controls and Mevinolin-treated embryos at stages 40. The number of embryos analyzed is indicated in the individual bars. Data were analyzed by Student׳s t-Test and the three asterisks indicate a significance of p<0.001. (E–I׳) Untreated controls and Mevinolin-treated embryos were processed for 3G8 and 4A6 immunohistochemistry at stage 40 (E–F׳) or whole mount in situ hybridization with β1-Na/K ATPase (G,G׳), Pmp70 (H,H׳) and Catalase (I,I׳) at stage 39. (J,J׳) Model for the role of lipid rafts in proximal tubule elongation in the wild-type situation (J) or in the absence of scp2 or upon treatment with Mevinolin (J׳). See discussion for details. Image published in: Cerqueira DM et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cav1.L bscl3, Cav-1, cav1-a, cav1-b, caveolin-1, cgl3, vip21 X. laevis Throughout NF stage 40 pronephric kidney atp1b1.L atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase X. laevis Throughout NF stage 39 pronephric kidney pronephric duct abcd3.L pmp70 X. laevis Throughout NF stage 39 pronephric kidney cat.2.L catalase X. laevis Throughout NF stage 39 pronephric kidney Image source: Published Larger Image Printer Friendly View

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