Xenbase Image ID: 147689

Fig. 3. CRISPR injected embryos phenocopy established mutant or MO knockdown phenotypes in the F0 generation. Left panel=WT embryo, Middle panel=MO injected embryo, Right panel=CRISPRp injected embryo. (a) Foxj1 CRISPRp – Immunohistochemistry of stage 28 embryos with acetylated tubulin to mark surface cilia. (b) Pax8 CRISPRp – in situ hybridization of stage 38 embryos with pax2 probe to stain kidney tubule. Black arrow points to pronephros. Image published in: Bhattacharya D et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pax2 LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. tropicalis Throughout NF stage 37 and 38 otic vesicle pronephric kidney brain midbrain-hindbrain boundary hindbrain optic stalk pronephric nephrostome Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + foxj1 CRISPR + NF28 (immunohistochemistry) fig.3.a Disease facial neoplasm autoimmune disease allergic rhinitis Xtr Wt + foxj1 MO + NF28 (immunohistochemistry) fig.3.a Disease facial neoplasm autoimmune disease allergic rhinitis Xtr Wt + pax8 CRISPR + NF37/38 (in situ hybridization) fig.3.b Anatomical Phenotype abnormal development of pronephric kidney abnormal pronephric kidney morphology Xtr Wt + pax8 MO + NF37/38 (in situ hybridization) fig.3.b Anatomical Phenotype abnormal development of pronephric kidney abnormal pronephric kidney morphology Larger Image Printer Friendly View

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