Xenbase Image ID: 157758

Fig. 2. Whole-mount in situ hybridization. Whole mount in situ hybridization with XlrpS1c was performed on Xenopus embryos at different developmental stages. (A) Neurula (stage 20), lateral view; (B) Early tailbud (stage 23) dorsal view; (C) Late tailbud (stage 27); (D, E) Hatching (stage 32), lateral view; (F) dorsal view of tailbud and hatching embryos; (G) Swimming tadpole (stage 35); (H) Swimming tadpole (stage 35 with double color in situ for XlrpS1c (red) and xJ1 (blue). (I, J) Whole mount in situ hybridization was performed on hatching stage (stage 32) embryos with ribosomal protein cDNA S22 (I), L1 (J, upper embryo) and L5 (J, lower embryo). (K) Negative control with sense XlrpS1c probe; early tailbud (stage 23) and late tailbud (stage 27), lateral views. so, somite; ov, optic vesicle; ba, branchial arches; cg, cement gland; ot, otic vesicle; he, heart; nm, nephrogenic mesoderm; hg, hatching gland. Arrowheads point to cement gland. Magnifications are as follows: A, B (30×); C, D, I, J (15×); E, F (10×); G, H (20×). Image published in: Scholnick J et al. (1997) Copyright © 1997. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rps3.L rps3-a, rps3-b, XlrpS1c X. laevis Throughout NF stage 35 and 36 somite pharyngeal arch eye intermediate mesoderm otic vesicle pronephric kidney head rps3.L rps3-a, rps3-b, XlrpS1c X. laevis Sometime during NF stage 20 to NF stage 23 somite optic vesicle head rps3.L rps3-a, rps3-b, XlrpS1c X. laevis Throughout NF stage 27 somite otic vesicle eye pharyngeal arch head rps3.L rps3-a, rps3-b, XlrpS1c X. laevis Throughout NF stage 32 somite pharyngeal arch head otic vesicle eye intermediate mesoderm pronephric kidney xa-1.L XA, xJ1 X. laevis Throughout NF stage 35 and 36 cement gland eye head pharyngeal arch hatching gland rpl4.L rpl1, rpl4-a, rpl4-b X. laevis Throughout NF stage 32 somite head pharyngeal region intermediate mesoderm eye brain rpl5.L rpl5-a, rpl5-b X. laevis Throughout NF stage 32 intermediate mesoderm brain head pharyngeal region eye rps15a.L rps22 X. laevis Throughout NF stage 32 eye intermediate mesoderm pharyngeal region head brain otic vesicle Image source: Published Larger Image Printer Friendly View

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