XB-IMG-173126
Xenbase Image ID: 173126
Fig. 1
Visualization of paired-type homeobox gene expression in the developing head skeleton of tailbud stage Xenopus laevis embryos. Whole-mount in situ hybridization was performed on embryos fixed at stages 28–30 (panels a–d) or 34–36 (panels e–h) using antisense riboprobes for alx1 (a, e), alx4 (b, f), prrx1 (c, g), or prrx2 (d, h). Specific stages are indicated in the lower right corner of each panel. Gene-specific antisense riboprobes were synthesized by in vitro transcription (Ambion MEGAscript kit) using specific cDNAs generated by 3′-RACE (Clontech SMARTer RACE Kit) using total RNA isolated from mixed neurula stage embryos using degenerate primers encoding VQVWFQN (5′-GTN CAR GTN TGG TTY CAR AAY-3′). Anatomical features are indicated in panel G: C—cement gland; E—eye; F—frontonasal process; Op—olfactory placode/pit; Ov—otic vesicle; P—pharyngeal arch Image published in: El-Hodiri HM and Kelly LE (2018) Copyright © 2018. Reprinted by permission from Springer Customer Service Centre GmbH: Copyright Springer Nature.
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