Xenbase Image ID: 175632

Supplemental Figure 4. Validation of l1cam F0 CRISPR mutant. (a) DNA gel showing the PCR product for each l1cam F0 CRISPR mutant and control, before and after cutting with T7 endonuclease I. Mid sagittal OCT image of control and two independent l1cam F0 CRISPR mutants, the later showing stenosis of the cerebral aqueduct (red arrowheads). (b) At stage 28 l1cam mRNA expression can be seen in the eye, brain, spinal cord and the notochord using in-situ hybridization. At stage 45 it is weakly expressed in forebrain but a strong expression is seen in the mid and hind brain region. (c) Immuno-fluorescence against l1cam protein shows loss of the signal in mutant as compared to the control stage 45 brain. Image published in: Date P et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue l1cam X. tropicalis Throughout NF stage 28 eye brain spinal cord notochord l1cam X. tropicalis Throughout NF stage 45 forebrain midbrain hindbrain l1cam X. tropicalis Throughout NF stage 45 brain Image source: Published Larger Image Printer Friendly View

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