XB-IMG-1842
Xenbase Image ID: 1842
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Figure 2. Spatial and temporal analysis of ashwin expression. Dorsal is toward the top, unless otherwise indicated. A: Stage (st.) 10; ashwin is expressed throughout the dorsal circumblastoporal zone. An arrow indicates the dorsal lip. B: A st. 12, dorsal view; expression is detected throughout the dorsal ectoderm and marginal zone. C: St. 12, ventral view; expression is excluded from the ventral hemisphere except for the circumblastoporal ring. D: Embryo hybridized with a sense probe. E: A st. 18, dorsal view; expression is visible in the neural ectoderm. F: A st. 18, frontal view reveals expression in anterior neural folds. G: A st. 18, midline sagittal dissection; expression is strongest in anterior and posterior neural domains but also is detected in the notochord and prechordal plate. H-K: At st. 22. H: Expression is detected throughout the developing nervous system and optic vesicles. I: Sense probe hybridization. J: Frontal view. K: Forebrain dissection; expression is detected in the ventral aspect of the brain and throughout the eye anlagen. L: At st. 26; ashwin is expressed throughout the central nervous system and in the tail bud. M: At st. 36; ashwin is expressed strongly in the brain and moderately in the posterior axial region. D, dorsal; V, ventral; A, anterior; P, posterior. N: Northern analysis of ashwin expression in Xenopus embryos at various embryonic stages. Each lane shows RNA isolated from two embryos at each of the stages indicated. The ashwin transcripts were visualized using a radiolabeled PCR probe. Two transcripts at 1.5 and 2.1 kb are expressed both maternally and zygotically. Levels of 18S and 28S rRNA are included to indicate the total amount of RNA loaded in each lane. Image published in: Patil SS et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons.
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