XB-IMG-23741
Xenbase Image ID: 23741
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Fig. 4. SRp38 inhibits the activity of neurogenin but not that of neuroD. (A) SRp38 blocks neurogenin induction of neuroD. Stage 15 lateral view, neuroD in situ hybridization. Control embryo: normal expression pattern of neuroD (in trigeminal ganglia indicated by red arrowhead). Expression of 500 pg SRp38 inhibits neuroD expression. However, expression of 250 pg of neurogenin induces ectopic expression of neuroD, while co-expression of SRp38 with neurogenin inhibits induction of neuroD (right embryo). (B) Ectodermal explant RT-PCR. SRp38 blocks neurogenin induction of NCAM, neuronal β-tubulin, synaptobrevin II and neuroD. Analysis of animal caps expressing either 500 pg of SRp38 and/or 250 pg neurogenin as indicated. EF1α is a loading control and muscle actin (mActin) controls for mesodermal contamination. (C) SRp38 does not block neuroD induction of synaptobrevin II (sybII). Stage 25 embryos stained for sybII. Control embryo: normal expression pattern of sybII in trigeminal ganglia and neurons. Expression of 500 pg SRp38 inhibits sybII expression. Ectopic expression of 250pg of neuroD induces ectopic expression of for sybII, while co-expression of SRp38 with neuroD does not inhibit neuroD induction of sybII. (D) Simplified schematic of neurogenic cascade. In the neural plate, neurogenin activates transcription of several downstream genes, including neuroD. neuroD then induces genes characteristic of neuronal differentiation such as neuronal β-tubulin and synaptobrevin. Notch signaling can inhibit the neurogenic effects of neurogenin but not neuroD. Image published in: Liu KJ and Harland RM (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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