Xenbase Image ID: 75970

Fig. 2. Differential expression of Gadd45a, Gadd45b and Gadd45g during Xenopus embryogenesis. (A–C) qPCR analyses showing the mean concentration normalized to GAPDH of Xenopus Gadd45a (A), Gadd45b (B) and Gadd45g (C). (D) Quantification of relative transcript levels of Xenopus Gadd45a, Gadd45b and Gadd45g in tadpole stage (st. 32) embryos. (E–S) Whole mount in situ hybridization expression analyses. (E–H) Gadd45a expression in lateral view of whole (E) and sagitally halved (F) gastrula stage (st. 10.5) embryos, dorsal is right. (G) Anterior and (H) dorsal view of late neurula stage (st. 18) embryo. (I and J) Gadd45b expression in anterior view of stage 18 embryo using antisense (I) and sense control probe (J). (K and L) Gadd45g expression in anterior view (K) and dorsal view (L) of stage 18 embryo. (M–P) Expression analysis of stage 18 embryos cut transversally with the probes indicated. (Q–S) Lateral view of tadpole stage (st. 32) embryos hybridized with probes for Gadd45a (Q), Gadd45b (R) and Gadd45g (S). The cement glands are outlined by dashed lines in (I–K). Key: (ba) branchial arches, (bp) blastopore, (cg) cement gland, (ey) eye, (fb) forebrain, (hb) hindbrain, (ht) heart field, (mes) mesoderm, (mb) midbrain, (nf) neural fold, (np) primary neuron precursors, (nt) neural tube, (pd) proctodeum, (pn) pronephros, (so) somites, (tg) trigeminal placode. Image published in: Kaufmann LT and Niehrs C (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gadd45a.S gadd X. laevis Throughout NF stage 10.5 ectoderm mesoderm gadd45a.S gadd X. laevis Throughout NF stage 18 ectoderm neuroectoderm cement gland primordium paraxial mesoderm gadd45a.S gadd X. laevis Throughout NF stage 32 proctodeum pronephric kidney pronephric duct endoderm head region gadd45b.L X. laevis Throughout NF stage 18 ectoderm gadd45b.L X. laevis Throughout NF stage 32 head region mesenchyme pharyngeal arch mandibular arch hyoid arch branchial arch neural tube anterior neural tube gadd45g.L XGadd45-gamma, xgadd45g X. laevis Throughout NF stage 18 trigeminal placode neural groove neural plate chordal neural plate cardiac mesoderm pre-chordal neural plate neuroectoderm cardiac progenitor cell epibranchial placode facial epibranchial placode vagal epibranchial placode gadd45g.L XGadd45-gamma, xgadd45g X. laevis Throughout NF stage 32 proctodeum midbrain hindbrain anterior neural tube forebrain telencephalon eye heart pronephric kidney trigeminal ganglion myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 18 paraxial mesoderm somite trunk somite sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 18 neuroectoderm neural plate neural fold Image source: Published Larger Image Printer Friendly View

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