Xenbase Image ID: 76916

Figure 3. Coexpression of XTcf-3 and putative XTcf-3 target genes. In situ hybridization reveals synexpression of XTcf-3, HMGN1, HMGN2, HMGX and XCIRP throughout early embryonic development. (A) At neurula stages all five genes are co-expressed and demarcate the anterior border of the neural plate (arrowhead). The main expression of HMGX is found in more posterior neural tissue (arrow) adjacent to the XTcf-4 expression field. The coexpression persists during early embryogenesis and is found in tailbud stages in head structures, including brain, eye, otic vesicle and branchial arches. (B) During gastrulation, XTcf-3 and XCIRP are co-expressed at the dorsal blastopore lip (arrowhead upper panel). The higher magnification reveals that both genes are expressed in the involuting mesoderm (anterior most mesoderm is indicated by a red arrowhead). In the endoderm (black arrowhead) underlying the involuting mesoderm, neither XTcf-3 nor XCIRP are expressed. While XTcf-3 is predominantly localized in the mesoderm (black arrow), the highest expression of XCIRP is found in the ectoderm (red arrow). But still substantial amounts of XCIRP RNA are detected in the mesoderm (black arrow). In anterior transversal sections of stage 18 embryos (lower panel) mRNA of both, XTcf-3 and XCIRP, is located in the neuroectoderm (arrowhead). While XTcf-3 is additionally found in the paraxial mesoderm (arrow), the most prominent XCIRP staining apart from the neural plate is located in the epidermis. (C) A higher magnification of transversal sections shows that following XTcf-3 morpholino injection (Tcf3Mo) XCIRP is strongly reduced in the neural plate (arrow) at the injected side (marked with an asterisk). Image published in: van Venrooy S et al. (2008) Copyright © 2008 van Venrooy et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tcf7l1.L tcf3c, tcf3d, tcf7l1-a, tcf7l1-b, XTCF-3, xtcf3 X. laevis Throughout NF stage 14 neural plate pre-chordal neural plate chordal neural plate paraxial mesoderm tcf7l1.L tcf3c, tcf3d, tcf7l1-a, tcf7l1-b, XTCF-3, xtcf3 X. laevis Throughout NF stage 28 eye visceral pouch hindbrain tail bud tcf7l1.L tcf3c, tcf3d, tcf7l1-a, tcf7l1-b, XTCF-3, xtcf3 X. laevis Throughout NF stage 10.5 involuting marginal zone mesoderm ectoderm upper blastopore lip involuted dorsal mesoderm tcf7l2.L LOC108697306, tcf4, tcf4e, XTCF-4 X. laevis Throughout NF stage 14 chordal neural plate neural plate neuroectoderm anterior tcf7l2.L LOC108697306, tcf4, tcf4e, XTCF-4 X. laevis Throughout NF stage 28 forebrain-midbrain boundary forebrain diencephalon hmgn1.L hmg14, hmgn1-a, hmgn1-b X. laevis Throughout NF stage 14 eye optic field chordal neural plate border hmgn1.L hmg14, hmgn1-a, hmgn1-b X. laevis Throughout NF stage 28 eye retina pronephric mesenchyme pronephric tubule hindbrain forebrain visceral pouch olfactory placode spinal cord tail bud hmgn2.L HMG-17 X. laevis Throughout NF stage 14 eye optic field chordal neural plate posterior placodal area lateral plate mesoderm hmgn2.L HMG-17 X. laevis Throughout NF stage 28 midbrain hindbrain intermediate mesoderm pharyngeal arch mandibular arch hyoid arch branchial arch retina hmgb2.S HMG-X, MGC52578 X. laevis Throughout NF stage 14 chordal neural plate anterior neural fold optic field hmgb2.S HMG-X, MGC52578 X. laevis Throughout NF stage 28 tail bud head region brain hindbrain eye cirbp.L cirbp-a, cirbp-b, cirbp2, cirp, cirp1, CIRP2, Xcirp, xCIRP2 X. laevis Throughout NF stage 14 neural plate cirbp.L cirbp-a, cirbp-b, cirbp2, cirp, cirp1, CIRP2, Xcirp, xCIRP2 X. laevis Throughout NF stage 28 pharyngeal arch mandibular arch hyoid arch branchial arch eye retina otic vesicle hindbrain spinal cord tail bud glomeral mesenchyme cirbp.L cirbp-a, cirbp-b, cirbp2, cirp, cirp1, CIRP2, Xcirp, xCIRP2 X. laevis Throughout NF stage 10.5 ectoderm upper blastopore lip involuted dorsal mesoderm Image source: Published Larger Image Printer Friendly View

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