Xenbase Image ID: 83604

Fig. 4. Whole-mount in situ hybridization analysis of XdrgmRNA distribution in Xenopus embryos. Embryos in A through E were hybridized with an Xdrg antisense probe. No hybridization to a control sense probe was detected under similar conditions (data not shown). (A! Dorsal and(B! lateral views of the stage 17 (neurula) embryos showing expression in neural folds (nf). IC) A stage 22 embryo showing expression in developing brain (br), otic vesicle (ov) and the neural tube (ntJ. Note the strong signals In the anterior and posterior regions of the CNS. (D,E) A stage 32 embryo showing expression In various head structures (brain. br: eye, ey; otic vesicle, ov), branchial arches-like structures (ba) and spinal cord. As a positive control, in (F), a stage 22 embryo was hybridized fa an antisense probe derrved from the Xenopus N-CAM cDNA (Kintner and Melton. 1987). under conditions similar to those used for the Xdrg probe. Image published in: Kumar S et al. (1993) Copyright © 1993. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 22 nervous system central nervous system optic vesicle neural tube posterior neural tube anterior neural tube drg1.L drg, xdrg1 X. laevis Throughout NF stage 17 dorsal neural fold anterior neural fold neural plate pre-chordal neural plate neuroectoderm drg1.L drg, xdrg1 X. laevis Throughout NF stage 22 posterior neural tube posterior neural tube anterior neural tube otic placode otic region brain drg1.L drg, xdrg1 X. laevis Throughout NF stage 32 nervous system central nervous system brain spinal cord eye forebrain midbrain hindbrain midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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