Figure 9. Immunostaining of activated Smads across the dorsoventral axis. Immunostaining of activated Smads across the dorsoventral axis. Stage 10+ embryos were fixed and bisected for immunohistochemistry. Dorsoventral orientation was determined by the dorsal blastopore lip, which is indicated with an arrow in (A-D). Embryo diameter is approximately 1 mm. Immunostaining with anti-phosphoSmad1 antibody was carried out with uninjected embryos (A) or embryos injected marginally with noggin (100 pg/embryo) (B) or BMP4 (500 pg/embryo) (C). AntiphosphoSmad2 immunostaining was performed with uninjected embryos (D) or embryos injected marginally with delta1XAR1 (1 ng/embryo) (E) or activin (50 pg/embryo) (F).
Image published in: Faure S et al. (2000)
Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| smad1.L | MLP, smad-1, smad1-a, smad1-b, XMad, Xmad1, Xsmad1 | X. laevis | Throughout NF stage 10.5 | mesoderm endoderm ventral ventral marginal zone vegetal yolk mass |
| smad2.S | smad-2, XSmad2 | X. laevis | Throughout NF stage 10.5 | mesoderm upper blastopore lip endomesoderm endoderm involuting marginal zone dorsal ventral vegetal yolk mass |
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