Fig. 5. Comparison of XSip1, XCtBP, and XCtBP1 expression during embryogenesis. (A) RT-PCR analysis of XCtBP, XCtBP1, and XSip1 expression at the indicated embryonic stages. Histone H4 was used as a loading control. Note that both XCtBP and XCtBP1 RNA are detected throughout development, while XSip1 transcripts accumulate at the start of zygotic transcription. (B) Whole-mount in situ hybridization of XCtBP, XCtBP1, and XSip1 at the indicated stages. All embryos are shown with anterior toward the right. (C) XCtBP expression in a horizontal section of a stage 18 embryo with a blow-up showing the restricted XCtBP expression in the deep sensorial layer of the ectoderm. Transversal sections of the neural tube at the level of the hindbrain of stage 31 embryos are shown at the right. Note that XCtBP and XCtBP1 have similar expression patterns during early embryonic development and are co-expressed with XSip1 in the developing neural tissue, XCtBP being expressed earlier and at a higher level than XCtBP1. Later, XCtBP and XCtBP1 have distinct expression domains within the developing neural tube. Abbreviations: mz, marginal zone; vz, ventricular zone; pn, pronephros; ba, branchial arches; nc, neural crest; nt, neural tube; pm, posterior mesoderm; sl, sensorial layer of the ectoderm. St. 11 embryos: dorso-vegetal view, anterior right; St. 13 embryos: dorsal view, anterior right; St. 25 and 31 embryos: lateral view, anterior right.
Image published in: van Grunsven LA et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| zeb2.S | sip1, smad interaction protein 1, XSip1, XZfhx1b, zfhx1b | X. laevis | Throughout NF stage 25 | hindbrain spinal cord midbrain optic field |
| ctbp1.L | xctbp1 | X. laevis | Throughout NF stage 25 | optic vesicle |
| ctbp1.L | xctbp1 | X. laevis | Throughout NF stage 31 | branchial arch eye forebrain midbrain hindbrain spinal cord |
| ctbp2l.S | X. laevis | Throughout NF stage 13 | mesoderm presumptive paraxial mesoderm neural plate | |
| ctbp2l.S | X. laevis | Throughout NF stage 25 | optic field | |
| ctbp2l.S | X. laevis | Throughout NF stage 31 | pronephric kidney branchial arch eye head mesoderm ventricular zone | |
| ctbp2l.S | X. laevis | Throughout NF stage 11 | mesoderm | |
| ctbp2l.S | X. laevis | Throughout NF stage 18 | mesoderm neural tube neural crest sensorial layer of neurectoderm paraxial mesoderm | |
| zeb2.S | sip1, smad interaction protein 1, XSip1, XZfhx1b, zfhx1b | X. laevis | Throughout NF stage 11 | ectoderm |
| zeb2.S | sip1, smad interaction protein 1, XSip1, XZfhx1b, zfhx1b | X. laevis | Throughout NF stage 13 | neuroectoderm neural plate |
| zeb2.S | sip1, smad interaction protein 1, XSip1, XZfhx1b, zfhx1b | X. laevis | Throughout NF stage 31 | eye hindbrain spinal cord branchial arch trigeminal nerve vagus nerve |
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