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XB-PHENO-21577

Xla Wt + ptf1a-GR + CHX + DEX, Hanotel J et al. (2014)

Experiment + Assay: Xla Wt + ptf1a-GR + CHX + DEX + NF15-16 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(ptf1a.L-GR) ptf1a.S
ptf1a.L
 NF stage 2 (2-cell) blastomere
chemical dexamethasone
NF stage 12.5 whole organism
chemical cycloheximide
NF stage 12.5 whole organism
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 15 to NF stage 16  
Expression Phenotypes (2):
mislocalised prdm13.L expression in neural plate
increased amount prdm13.L expression in neural plate
Source: B
Article: XB-ART-48401, PubMed