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XB-PHENO-21725

Xla Wt + Mmu.ascl1, Wylie LA et al. (2015)

Experiment + Assay: Xla Wt + Mmu.ascl1 + NF18-19 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(Mmu.ascl1) ascl1.L
ascl1.S
 NF stage 2 (2-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 18 to NF stage 19  
Expression Phenotypes (4):
mislocalised hand2.L expression in anterior placodal area
increased amount phox2a.S expression in anterior placodal area
increased amount th.S expression in anterior placodal area
increased amount tubb2b.S expression in neural plate border
Source: Fig.6.a,b
Article: XB-ART-50246, PubMed