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Xla Wt + VP16-Flag-prdm12, Thélie A et al. (2015)

Experiment + Assay: Xla Wt + VP16-Flag-prdm12 + NF32 (in situ hybridization)
Background: Xla.Outbred
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(VP16-Flag-prdm12) prdm12.S
NF stage 2 (2-cell) blastomere
Assay Type Assay Stages Antibody
in situ hybridization NF stage 32  
Expression Phenotypes (3):
decreased amount en1.L expression in neural tube
mislocalised nkx6-1.L expression in neural tube
increased amount nkx6-1.L expression in neural tube
Source: Fig.5.c
Article: XB-ART-51355, PubMed