XB-PHENO-22524
Xla Wt + gli2 MO + gli3 MO, Rankin SA et al. (2016)
| Experiment + Assay: |
Xla Wt + gli2 MO + gli3 MO +
NF33/34 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
gli2 MO1
|
gli2.S
gli2.L
|
NF stage 3 (4-cell)
|
blastomere
|
|
morpholino injection
|
gli3 MO2
|
gli3.L
gli3.S
|
NF stage 4 (8-cell)
|
blastomere
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 33 and 34
|
|
|
|
|
Expression Phenotypes (5):
|
|
decreased amount
nkx2-1
expression in forebrain
|
|
decreased amount
nkx2-1
expression in foregut endoderm
|
|
decreased amount
nkx2-1
expression in lung primordium
|
|
decreased amount
nkx2-1
expression in thyroid primordium
|
|
decreased amount
wnt2
expression in lateral plate mesoderm
|
|
