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XB-PHENO-23966

Xla Wt + bmp4 + CHX + animal cap explant, Lee H et al. (2020)

Experiment + Assay: Xla Wt + bmp4 + CHX + animal cap explant + NF10 (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection bmp4 (Xla) bmp4 (Xla)
 NF stage 3 (4-cell) animal blastomere
chemical cycloheximide
NF stage 8 animal cap
explant  
NF stage 8 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 10 to NF stage 10  
Expression Phenotypes (2):
increased amount szl.L expression in animal cap
increased amount ventx1.2.L expression in animal cap
Source: Fig. 3 c
Article: XB-ART-57519, PubMed