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Now that the [human and Xenopus] genomes are sequenced, interest is focused on the study of the proteome, which is the protein content of an organism, tissue, or cell. As a member of the Norm Dovichi group we are developing tools to study the proteome with either UPLC or two-dimensional capillary electrophoresis, coupled to either laser-induced fluorescence or mass spectrometry for detection. The group's long-term goal is to study protein expression in single cells and to determine how protein expression changes across a cellular population during cancer progression and during the development of an embryo. In the shorter term, we are developing tools to characterize post-translational modifications of proteins and to speed digestion and analysis of proteins.