Xenbase Image ID: 154127

Fig. 1. The expression of the P-TEFb complex components during Xenopus laevis development. Transcripts for the P-TEFb components were detected using NBT/BCIP and expression of CDK9.S, CDK9.L and CyclinT1 appeared blue in the sectioned neurula stage embryo. Sox10 was detected using fast red and fluorescence microscopy. (A) CDK9.S expression at stage 16 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Bi) 10× magnification section of a stage 16 embryo showing CDK9.S expression in the neural plate and neural plate border (blue) and Sox10 expression in the neural crest (red). (ii) 20× magnification section shows overlap of the blue CDK9.S expression with the red Sox10 expression. Expression of CDK9.S is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm (C) CDK9.L expression at stage 16 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Di) 10× magnification section of a stage 16 embryo showing CDK9.L expression in the neural plate and neural plate border (ii) 20× magnification section shows overlap of the blue CDK9.L expression with the red Sox10 expression. Expression of CDK9.L is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm (E) CyclinT1 expression at stage 15 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Fi) 10× magnification section of a stage 15 embryo showing CDK9.S expression in the neural plate and neural plate border (blue) and Sox10 expression in the neural crest (red). (ii) 20× magnification section shows overlap of the blue CDK9.S expression with the red Sox10 expression. Expression of CDK9.S is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm. Npb=Neural plate border, ba=branchial arches. Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cdk9.S c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 16 neural plate neural plate border neural crest cdk9.S c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 18 neural plate neural plate border neural crest cdk9.S c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 26 pharyngeal arch head brain cdk9.S c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 32 branchial arch head brain cdk9.L c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 16 neural plate neural plate border neural crest cdk9.L c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 18 neural plate neural plate border neural crest cdk9.L c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 26 pharyngeal arch head brain cdk9.L c-2k, cdc2l4, cdk9-a, cdk9-b, ctk1, pitalre, tak X. laevis Throughout NF stage 28 branchial arch head brain ccnt1.L X. laevis Throughout NF stage 15 neural plate neural plate border neural crest ccnt1.L X. laevis Throughout NF stage 18 neural plate neural plate border neural crest ccnt1.L X. laevis Throughout NF stage 26 pharyngeal arch brain ccnt1.L X. laevis Throughout NF stage 32 branchial arch brain sox10.L X. laevis Sometime during NF stage 15 to NF stage 16 neural plate border neural crest Image source: Published Larger Image Printer Friendly View

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