Xenbase Image ID: 45499

Fig. 3. XSPR-1 and XSPR-2 expression during early embryogenesis as detected by whole-mount in situ hybridization. Antisense RNA probes used for in situ hybridization and stages of embryogenesis (Nieuwkoop and Faber, 1967) are shown on the right and on the left of each panel, respectively. (A,C,E,G) Vegetal view; (B,D,F,H) parasagittal section; (C,D) double staining in situ hybridization of Xbra (red) with XSPR-1 (purple); (D) parasagittal section of the embryo shown in (C); (G,H) double staining in situ hybridization of Xbra (red) with XSPR-2 (purple); (H) parasagittal section of the embryo shown in (G); (I) double staining in situ hybridization of Krox20 (red) with XSPR-1 (purple), anterior view; (J) anterior transversal section; (K) double staining in situ hybridization of En2 (red) with XSPR-1 (purple), anterior view; (L) lateral view of the head of a tadpole embryo; (M) double staining in situ hybridization of Krox20 (red) with XSPR-2 (purple), dorso-anterior view; (N) XSPR-2, posterior transversal section; (O) double staining in situ hybridization of En2 (red) with XSPR-2 (purple), dorso-anterior view; (P) lateral view of a tadpole embryo; (Q) transversal section at the level of the otic vesicle; (R) transversal section at the level of the midbrain/hindbrain boundary; (S) parasagittal section through the tail tip region. Abbreviations: aet, archenteron; bc, blastocoel; br, blastocoel roof; cnh, chordaneural hinge; end, endoderm; ep, epidermis; ev, eye vesicle; eym, endodermal yolk mass; fb, forebrain; lm, lateral mesoderm; mbh, midbrain/hindbrain boundary; mc, mesencephalon; nc, notochord; nec, neuroenteric canal; ov, otic vesicle; pw, posterior wall; rb, remnants of blastocoel; rc, rhombencephalon; rv, rhombencephalic ventricle; s, somites; sln, sensorial layer of neuroectoderm; sm, somitogenic mesoderm; vym, vegetal yolk mass. Image published in: Ossipova O et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Throughout NF stage 10.5 mesoderm involuting marginal zone marginal zone ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Sometime during NF stage 11 to NF stage 11.5 marginal zone sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Throughout NF stage 16 sensorial layer of neurectoderm notochord lateral plate mesoderm neural plate sp5l.S gli1-2, spr-2, spr2, xspr-2, XSpr2 X. laevis Sometime during NF stage 32 to NF stage 66 chordoneural hinge tail bud posterior wall of neurenteric canal sp5.S spr1, xsp5, xspr-1 X. laevis Throughout NF stage 11.5 dorsal non-involuting marginal zone epithelium tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Sometime during NF stage 11 to NF stage 11.5 marginal zone involuted dorsal mesoderm mesoderm sp5.S spr1, xsp5, xspr-1 X. laevis Throughout NF stage 16 pre-chordal neural plate neural plate presumptive midbrain-hindbrain boundary en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 16 to NF stage 18 midbrain-hindbrain boundary neuroectoderm egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Sometime during NF stage 16 to NF stage 17 rhombomere R3 rhombomere R5 neural plate sp5.S spr1, xsp5, xspr-1 X. laevis Throughout NF stage 32 midbrain-hindbrain boundary midbrain otic vesicle Image source: Published Larger Image Printer Friendly View

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