Xenbase Image ID: 82534

Fig. 3. Effects of neural inhibitors and neural inducers on XXBP-1 expression. (I) Neural inhibitors and β-catenin morpholino expand the expression territories of XXBP-1. Embryos were injected dorsally at the four-cell stage with (A) 8 ng β-catenin morpholino, (B) 1 ng BMP-4 mRNA, (C) 80 pg Dlx3 or (F) 80 pg Msx1 mRNA, and were fixed at midgastrula stages for whole-mount in situ hybridization with XXBP-1 antisense RNA. (D) Uninjected embryos were UV irradiated at 302 nm for 45 s. The expression area of XXBP-1 was expanded compared with control embryos (E). Whole-mount in situ hybridization and fixation after staining were performed identically for all embryos to eliminate differences due to overstaining. (II) Gene expression in noggin or Zic3 animal caps. At stage 9, animal caps were dissected from uninjected embryos (AC) or embryos injected into all four blastomeres at the four cell stage with 160 pg Zic3 or 160 pg noggin mRNA. The expression of BMP-4, XXBP-1, Xvent-1, and NCAM was assayed by using RT-PCR from RNA isolated after sibling embryos reached stage 18. ODC served as a loading control. Noggin suppressed XXBP-1 and BMP-4 expression, but induced NCAM. Image published in: Zhao H et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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