Research Interests

Localized molecules and maternal signalling pathways in vertebrate development

Research Area

In many vertebrate eggs, fertilization initiates cortical/cytoplasmic rearrangements (cortical rotation), resulting in the transport of critical determinants to the future dorsal side. In the frog Xenopus, this event is mediated by microtubules and causes the localized activation of the Wnt signaling pathway dorsally. Wnts are key growth factors involved in many aspects of development and are often misregulated in cancers and other diseases. Similar cytoplasmic localizations and Wnt activation occur in many chordate embryos, suggesting a deeply conserved mechanism for patterning early embryos. Our lab is currently pursuing several main avenues of investigation: (1) identifying and characterizing the roles of asymmetrically-localized mRNAs and proteins in early dorsal axis formation, (2) elucidating mechanisms of microtubule assembly and orientation during cortical rotation and (3) determining how Wnt signaling is initiated and regulated on the dorsal side. We are addressing these questions using reverse-genetic antisense approaches in the amphibian Xenopus laevis. Additionally, we are beginning to explore bona fide genetic manipulation of these processes in the genetically-tractable diploid frog species, Xenopus tropicalis.

Current Members

Alumni

Cuykendall, Tawny N (Graduate Student)

Additional Information

Keywords: developmental biology, germ cells, germ layer, localized RNAs, Wnt, axis formation, Xenopus

Contact

Institution: University of Iowa

Address:
Developmental Biology
257 Biology Building
129 East Jefferson St.
Iowa City, IA
52242, USA

Web Page: http://biology.uiowa.edu/people/douglas-houston

Personal Phone: (319) 335-1316

General/Lab Phone: (319) 335-1317