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Fig. 2. XCRISP is expressed in the hatching gland. At stage 17 (A) the XCRISP positive cells are co-localised with XAG in two stripes outlining the neural plate. In later neurula, stages 23 (B) and 28 (C), with closure of the neural tube the two lines of hatching gland cells converge to a y-shaped pattern. XAG staining is overlaid by XCRISP, but visible in the XCRISP-negative cement gland (red). Expression of XCRISP persists till stage 32 (D) when still positive cells are detected in a shorter y-pattern on the head of the embryos. XCRISP expressing cells are detected in two patches directly on top of the neural tube in stage 23 (E), in stage 28 the two separate groups of cells have fused to a single structure (F). The temporal expression profile is confirmed by RT-PCR, which detects XCRISP from stage 19 to 35 (G). |
