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Fig. 5. XSufu is important for cement gland, neural crest, neuronal and paraxial mesoderm development. Embryos were injected together with nlacZ mRNA as tracer animally into one blastomere at the 2- or 4-cell stage. (A,A′,B,B′) Whole-mount in situ hybridization of early neurula embryos in anterior view. XSufu mRNA and XSufu-MO lead to a reduction of the cement gland marker XAG while a non-specific control-MO has no effect. (C–F,C′–F′,D′,F′) Late gastrulae in dorsal and tail bud embryo in lateral view. XSufu mRNA and XSufu-MO cause a reduction of the neural crest markers Slug and Snail. Co-injection of XSufu-MO and XSufu* mRNA reverts to normal Slug and Snail expression. (G,G′,H–H′) Early neurulae in dorsal view. XSufu mRNA causes slight expansion of the neuronal marker N-tubulin. XSufu-MO reduces N-tubulin expression. Normal N-tubulin expression is seen after co-injection of XSufu-MO with XSufu* mRNA. (I–I′) Tail bud embryos in dorsal view. XSufu-MO inhibits formation of MyoD-positive segmented somites (see also magnification in inset). Indicated effects were observed in: A′, 9/16; B, 40/45; B′, 7/7; C′, 70/70; D, 65/68; D′, 14/15; D′, 62/64; E′, 62/65; F, 13/13; F′, 19/19; F′, 30/33; G′, 66/81; H, 102/105; H′, 78/84; H′, 31/39; I, 15/20; I′, 17/17. |
