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Fig. 5. Cement gland induction in animal cap explants from X- bhh-injected embryos. (A) Uninjected tailbud embryo stained for expression of the cement gland marker XAG-1 by in situ hybridization (white arrow). (B) Histological section of a stage 25-equivalent animal cap explant from an uninjected embryo stained for XAG-1. (C) Histological section of stage 25-equivalent animal cap explant from a lacZ mRNA- injected embryo stained for XAG-1. (D) Animal caps from X-bhh-injected embryos, cultured until siblings reached stage 25 and stained for XAG-1. (E) Histological section of one of the explants shown in D demonstrating foci of cement gland formation (white arrowheads). (F) Histological section of a X-bhh-injected animal cap showing the densely packed columnar morphology characteristic of cement gland secretory cells (bracket). |
