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Figure S5 (related to Figure 7). Knockdown of Kdm2a/b regulates β-Catenin
target gene expression.
(A and B) β-Catenin knockdown showed rescuing effect on embryonic development
(A) and gene expression (B) in most embryos with simultaneous knockdown of
Kdm2a and 2b. In (A), 20 ng of ctrl MO, 10 ng of Kdm2aMO, 5 ng of Kdm2bMO,
and 5 ng of β-CatMO was injected. In (B), 30 ng of ctrl MO, 15 ng of Kdm2aMO, 10
ng of Kdm2bMO, and 10 ng of β-CatMO was injected.
(C) Knockdown of Kdm2a or 2b mediated the transcription of target genes of both
maternal and zygotic Wnt/β-Catenin and of genes marking A-P or D-V axis formation.
20 ng of ctrlMO or Kdm2aMO, and 10 ng of Kdm2bMO was injected. The stages of
control embryos are labeled. d: dorsal view; p: posterior view; a: anterior view.
(D) Simultaneous knockdown of Kdm2a and 2b led to synergistic regulatory effect of
genes marking A-P or D-V axis formation. The stages of control embryos are labeled.
d: dorsal view; p: posterior view; l: lateral view.
(E-H) The majority of embryos displayed changes in the expression of xnr3 (E),
ventx1.2 (F), hoxb9 (G) and xk81a1 (H), as shown in (D). |
