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FIG. 2. Cement gland expansion is limited to the anterior region of ectopic Xgsk-3 expression. Embryos were injected with Xgsk-3 and
b-galactosidase RNA in one cell at the two-cell stage, cultured until stage 20, and stained for b-galactosidase activity (red) and XAG-1
expression (purple). (A) Uninjected sides; dorsal is to the right and anterior is at the top. (B) Injected sides of the embryos in (A); dorsal is
to the left. Note that XAG-1 expression is limited to the anterior region of b-galactosidase activity. Occasional b-galactosidase expressing
cells on the uninjected side of the embryo indicate that the first cleavage does not always perfectly divide the embryo into left and right
halves. |
