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Experiment details for ag1

Michiue T et al. (2009) Assay

Xenopus galectin-VIa shows highly specific expression in cement glands and is regulated by canonical Wnt signaling.

Gene Clone Species Stages Anatomy
ag1.L laevis NF stage 13 ectoderm
ag1.L laevis NF stage 18 cement gland primordium , neural plate
ag1.L laevis NF stage 24 cement gland primordium
ag1.L laevis NF stage 27 hatching gland , cement gland primordium

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  Fig. 1. Spatial pattern of Xgalectin-VIa (A,B,C,J,K,L,M,N,O), XCG (D,E,F,Q) and XAG-1 (G,H,I,P) expression by whole-mount in situ ybridization. (A,D,G) Anterior views of the early neurula. Dorsal side is up. (B,E,H) Anterior views of the late neurula. (C,F,I) Lateral views of the tailbud. Xgalectin-VIa was expressed in a highly specific pattern in the cement gland region. (Jâ L) Spatial pattern of Xgalectin-VIa expression at late-tailbud (J) and tadpole (K,L) stages. CG, cement gland; eye, eye vesicle; OV, otic vesicle. (M,N) Histological section of (l). Sectioned regions are indicated in (l). (Oâ Q) Comparison of Xgalectin-VIa expression patterns (O) with those of XAG-1 (P) and XCG (Q) in the anterior region of tadpole-stage embryos. Xgalectin-VIa was expressed in hatching gland (HG; indicated with arrowhead), as observed for XAG-1.

Gene Clone Species Stages Anatomy
ag1.L laevis NF stage 18 cement gland , cement gland primordium

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  Fig. 3. Effect of canonical Wnt signaling on Xgalectin-VIa expression. (A) 500 pg of β-catenin (βcat) mRNA (lane 1), 250 pg (lane 2) of βcat mRNA, 500 pg of lacZ (lane 3), 20 ng of standard control morpholino antisense oligo (CMO; lane 4), 10 ng of β-catenin MO (βcatMO; lane 5), and 20 ng of βcatMO (lane 6) were injected into animal poles of 8-cell embryos, followed by RT-PCR analysis with cDNAs derived from these whole embryos. The order reflects the gradient of canonical Wnt signaling. Similar to XAG-1 and XCG, Xgalectin-VIa expression was decreased by βcat mRNA (lanes 1, 2) and increased by βcatMO (lanes 5, 6). (B–J) Effects of βcat mRNA or βcatMO injection on Xgalectin, XAG-1, and XCG expression were also examined by WISH. 500 pg of lacZ mRNA (B,E,H), 500 pg of lacZ mRNA and 500 pg of βcat mRNA (C,F,I), or lacZ mRNA and 20 ng of βcatMO (D,G,J) were injected into the animal pole region of 8-cell embryos. After active staining with RED-Gal at stage 18, WISH was carried out with probes for Xgalectin-VIa (B,C,D), XAG-1 (E,F,G), and XCG (H,I,J). In all cases, βcat mRNA injection caused loss of gene expression (C,F,I). Conversely, βcatMO injection induced ectopic expressions (D,G,J; arrowhead).