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Fig. 3. The proximal regulatory region mimics endogenous Xath5 retinal expression in the ciliary marginal zone. (A-C) Double in situ hybridization on retinal sections from stage 42 embryos comparing expression of the GFP transgene mRNA (red; A,B) as driven by the pG1X5-proximal construct to endogenous Xath5 mRNA (purple; B,C). (D) Unlike the RNA, GFP protein is found throughout the central retina and in many cell types at stage 42. Bracket marks the CMZ, which lacks GFP fluorescence. (E-G) Higher magnification images of the ciliary marginal zone (CMZ) from A-C showing cell-by-cell correlation of GFP (red) and Xath5 (purple) mRNAs. |
