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FIG. 3. Expression of XBrn3d and Xath5 overlaps in the ciliary marginal zone of the Xenopus retina. Double in situ hybridization was perform ed on stage 41 em bryos using an antisense DIG probe (XBrn3d) and an antisense fluorescein probe (Xath5). (A) Schem atic showing a cross section of the Xenopus retina. The retinal pigment epithelium is shown as a thick line. The retinal ganglion cell layer (RGC) is closest to the lens (L), while the photoreceptor layer (ON L) borders the pigm ent epithelium and the inner nuclear layer (IN L) is between. The ciliary marginal zone (CMZ) is the region of continued proliferation, in which the central CMZ is the portion closest to the differentiated retinal layers. (B) XBrn3d expression is restricted to the retinal ganglion cell layer and the central CMZ. (C) The composite im age of both the Xath5 and the XBrn3d staining shows double-labeled cells. (D) Xath5 is expressed in the CMZ. Double-labeled cells are indicated with brackets (B, D). |
