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Fig. 5. Co-expression of XSix3 and XneuroD. Double whole-mount in situ hybridisation detecting XSix3 (blue) and Xath5 (red, A) and XNeuroD (red, D).
Anterior to the left, dorsal (A, C, D, G, J, M), lateral (B, E, H, K, N) and frontal views (F, I). Stages as indicated. (A) Full overlap of expression Xath5 and
XSix3 detectable only in embryos weakly stained for XSix3. Compare A and J for XSix3. (D) No overlap of expression detected at stage 21. (G) Coexpression
first detectable at stage 24. Note that at this stage only scattered individual cells express XNeuroD in a broad XSix3 domain (arrow in inset in H). In
addition, the presumptive pineal organ expresses both genes (arrowhead in H and K, compare this to Fig. 1B). (J) At stage 28, co-expression broadens,
comprising almost the entire retina (arrowhead in L). (M) At stage 37, co-expression becomes restricted to marginal regions (arrowheads in O). (L, O)
Transversal sections at levels indicated in J and M, dorsal to the left, inset shows NeuroD single staining. |
